摘要
[目的]研究冬虫夏草(Cordyceps sinesis)菌丝体热水提物(CS-HWE)抗氧化活性及体外氧化损伤保护作用。[方法]采用铁离子还原法(FRAP)和H2O2氧化损伤的PC12细胞模型对CS-HWE抗氧化活性及对氧化损伤保护活性分别进行了测定。[结果]样品抗氧化活性为(158.03±5.39)μmolFe2+·g-1,相当于天然虫草提取物活性的75%。CS-HWE在50￣100mg·L-1时可明显提高PC12细胞对H2O2氧化损伤的保护作用,活性与天然虫草提取物相当。[结论]虫草菌丝体热水提物具有一定的抗氧化作用,可作为开发天然抗氧化药物的潜在资源。
[Objective]To investigate the activities of CS-HWE,the hot-water extracts derived from the liquid-cultured Cordyceps sinensis mycelia,on antioxidantion and oxidative injury protection.[Methods]The antioxidant activity was detected by using a Ferric Reducing Antioxidant Power assay（FRAP）. The oxidative injury protective effect was assessed by using a PC12 oxidative injury model treated with H2O2,and the survived cells were measured by using MTT assay.[Results]The FRAP assay showed the CS-HWE exerted an antioxidative activity,which was（158.03 ± 5.39）μmol Fe^2＋·g^-1. Its activity was about 75% of that of the whole nature Cordyceps extract was. CS-HWE at concentrations of 50-100 mg·L^-1 could significantly preserve viable PC12 cells after H2O2 treatment,and the protective effect was equivalent to the whole nature Cordyceps extract（P﹤0.05）.[Conclusion]The hot-water extract of Cordyceps sinensis fermentative mycelia possess notable antioxidative activities and has the potential to be used as an antioxidative agent.
出处
《现代预防医学》
CAS
北大核心
2007年第18期3433-3435,共3页
Modern Preventive Medicine
基金
上海市教育委员会科学项目(04DB20)
香港保健协会部分资助
