摘要
通过构建自杀质粒,用定向同源重组的方法,把改造的萤火虫萤光素酶报告基因Luc NF+替换布鲁氏菌S19疫苗株Bp26基因部分片段,构建出布鲁氏菌Bp26基因缺失突变株△S19-1。在此基础上,用同样的方法,敲除△S19-1上的毒力基因Bmp18,构建成△S19-2。用PCR与萤光素酶报告基因在布鲁氏菌中表达的方法进行验证,结果表明Bp26基因与Bmp18基因改造成功,△S19-2具有Bp26基因分子标记、Bmp18毒力基因缺失的特点。
A Bp26 gene mutated strain △S19-1 was constructed by replacing part of Bp26 gene of B. abortus with Luc NF^＋ gene, a gene amplified from Luciferase gene. And Bmp18 gene was deleted from △S19-1 to construct △S19-2. PCR and expression of Luciferase gene in B, abortus showed that △S19-2 with a molecular marker and virulence genes of Bp26 and Bmpl8 deleted was constructed successfully.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2007年第5期690-694,699,共6页
Chinese Journal of Veterinary Science
基金
军事医学科学院学科培育项目
