摘要
表达新疆绵羊布鲁氏菌外膜抗原蛋白OMP3b的表达蛋白OMP2b,探索其作为诊断抗原和亚单位疫苗的可能性。采用PCR方法,从新疆绵羊布鲁氏菌基因组DNA中扩增出omp2b基因片段,将该片段克隆于原核表达载体PET-28a(+),构建成重组质粒,经IPTG诱导,SDS-PAGE检测。结果表明,获得长约1083bp的PCR片段,序列分析结果与已知绵羊布鲁氏菌外膜蛋白OMP2b同源性达89.72%;SDS-PAGE检测表达产物,在相对分子质量39ku处有表达带。获得了新疆绵羊布鲁氏菌外膜蛋白OMP36的表达蛋白omp2b基因片段,并在大肠杆菌中实现了表达。
OMP2b antigen protein of OMP36 from out membrane protein of brucella ovis in Xinjiang was expressed in order to study the probability for it as molecular vaccine and diagnostic antigen. OMP2b protein gene fragment was amplified from brucella ovis in Xinjiang genomic DNA through PCR. The fragment was identified and cloned into prokaryotic expression vector PET- 28a （＋）. Then iducement expression was used to express OMP2b antigen protein by IPTG. （1）An about 1089bp length PCR product was obtained,the homology was 89. 72%. （2） An expression band about 36 ku was found, conclusion, we obtained omp2b gene fragment and expressed the protein successfully in Escherichia coli .
出处
《动物医学进展》
CSCD
2005年第8期80-83,共4页
Progress In Veterinary Medicine
基金
新疆兵团博士基金(兵博02)
