摘要
目的:采用手十二井穴刺络放血方法干预抑郁大鼠,观察大鼠血浆细胞因子的变化以及行为学的特点。方法:实验2005-11/2006-02在大连医科大学实验动物中心及附属第二医院实验中心完成。①实验材料:普通级成年wistar大鼠32只,体质量180～220g,雌雄各半。②实验分组:将大鼠随机分为正常对照组、模型组、百优解组和刺络放血组,每组8只。③实验干预:正常对照组大鼠不予任何刺激和治疗。其他组大鼠孤养并接受21d各种不同刺激后,模型组不予任何治疗,百优解组按2.8mg/kg灌胃,1次/d;刺络放血组选用大鼠双侧前肢趾端的十二井穴刺络放血,隔日1次,共11次,出血量为每穴1滴。④实验评估:对比各组大鼠行为学指标(采用Open-Field法)及血浆中白细胞介素1β、白细胞介素6的差异。结果:32只大鼠全部进入结果分析。①各组大鼠血浆中白细胞介素1β、白细胞介素6水平变化:正常组、百优解组、刺络放血组与模型组大鼠比较,白细胞介素1β、白细胞介素6差异均有显著性(P<0.01);百优解组、刺络放血组与正常组比较,以及刺络放血组与百优解组比较,白细胞介素1β、白细胞介素6差异无显著性(P>0.05)。②各组大鼠Open-Field行为得分:正常组与模型组动物比较,水平得分与垂直得分差异均有显著性(P<0.01);刺络放血组、百优解组与模型组比较,水平得分与垂直得分差异均有显著性(P<0.05～0.01);刺络放血组与百优解组的得分差异无显著性(P>0.05)。结论:手十二井穴刺络放血后,抑郁大鼠模型血浆中的白细胞介素1β、白细胞介素6的含量降低,行为学能力改善,从而产生抗抑郁的作用。
AIM： TD observe the antagonism against depression of blood-letting puncture at hand twelve Jing-acupoints in rats, and investigate the variations of plasma cytokines and behaviors. METHODS： From November 2005 to February 2006, this experiment was conducted cooperatively with the Animal Experimental Base of Dalian Medical University and the Central Laboratory of the Second Hospital Attached to Dalian Medical University. ①Thirty-two adult wistar rats of common grade and half gender were used in this study, aged 180- 220 g.②These rats were randomly allocated to four groups （n =8）： normal control group, model group, fluoxetine group and blood-letting puncture group.③The rats of normal control group were fed routinely without stimulation and treatment. While other rats were singly housed and exposed to different stressors for 21 days, those of model group received no treatment, those of fluoxetine group were injected with 2.8 mg/kg drugs intragastrically, once per day, and those of puncture group were pricked on twelve Jing-acupoints of rat bilateral forelimb toes for blood-letting, one drop for one acupoint, once every two days, for totally 11 times. ④The behavior index （by Open-Field method） and the levels of interleukin （IL）-1β, IL-6 in plasma of depression rats were compared, respectively. RESULTS： All of 32 rats were involved in the result analysis.①Compared with the model group, the levels of IL-1β and IL-6 in plasma of rats in normal control group, fluoxetine group and puncture group all had significant differences （P 〈 0.01）; There was no significant difference between puncture group, model group and control group, between puncture group and fluoxetine group （P 〉 0.05）.②Compared with the model group, the horizontal score and perpendicular score of control group had significant differences （P 〈 0.01）, these scores of puncture group and fluoxetine group also had significant differences （P 〈 0.05-0.01）; No significant difference was found between the punct
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第36期7161-7164,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
