摘要
目的采用三种方法建立兔单肺通气模型并比较其效果。方法日本大耳白兔30只,随机分为3组(即A、B、C组)各10个,分别采用自制双腔气管导管法、左主支气管结扎法和插管过深法。呼吸机通气参数为:FiO21.0,RR 40/min,VT 10 mL/kg。单肺通气2 h后恢复双肺通气。记录各组单肺通气实施的一次成功率、总成功率、从气管切开开始到单肺通气实施所需要的时间、动物失血量。实验结束后开胸测量兔气管、左主支气管、右主支气管的长度和内径。结果各组进入实验的动物数分别为10、6、8只。与B、C组比较,A组一次成功率和总成功率高,所需时间明显较少(P<0.01),且出血量明显少于B组(P<0.01)。结论采用自制双腔气管导管能迅速有效的建立单肺通气模型,是用于研究与单肺通气相关病理生理机制的理想模型。
Objective The purpose of this study was to compare three types of one-lung ventilation （OLV） models in rabbits. Methods 30 rabbits weighing 2.3 ±0.2 kg were anesthetized with ketamine （25 mg/kg i. m. ） and were ventilated via tracheotomy tubes at a rate of 40/min with a tide volume of 10 mL/kg with pure oxygen and divided randomly into three groups. In group A （n = 10） OLV was carried out by a double-lumen endotracheal tube made by ourself. In group B thoracotomy was performed and the left main bronchus was completely clamped with a clip,so OLV was carried out. In group C tracbeotomy tube was pushed into the right main bronchus to perform OLV. The first time and overall success rates, the time from tracheotomy beginning to OLV beginning and volume of bleeding were recorded. The rabbit trachea was measured. Results In group A the first time and overall success rates were higher than those in group B and C. The numbers of rabbits in the three groups coming into experiment were 10,6 and 8, respectively. In group A the time from tracheotomy beginning to OLV beginning was significantly shorter than that of group B and C（ P 〈 0.01 ）. Compared with group B the bleeding volume was significantly lower than that in group A P 〈 0.01 ）. Conclusion The rabbit model of OLV generated by the artificial double-lumen endotracheal tube is convenient,satisfactory and safe. This model is suitable for investigation of pathophysiological mechanism associated with OLV.
出处
《中国实验动物学报》
CAS
CSCD
2007年第4期245-248,共4页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金项目(编号:30571787)
关键词
兔
单肺通气
模型
动物
Rabbit
One-lung ventilation
Models, animal
