摘要
利用S1-8-RNase氨基酸保守序列‘FTQQYQ’和‘IIWPNV’设计兼并引物，对白梨4个品种冬黄、新梨一号、红皮酥和博山池的基因组DNA进行PCR扩增。PCR产物限制性酶切分析显示：新梨一号和冬黄各含有一个S8-等位基因。DNA序列测定和生物信息学分析表明，这4个品种中含有与已报道的S1-19等位基因有差异的S基因，经分析鉴定为新的S等位基因，分别命名为S20-、S22-、S26-、S27-等位基因（GenBank登录号分别为：AY250988、AY250990、AY339396、AY339397）。冬黄、新梨一号、红皮酥和博山池的S基因型分别为S8S20、S8S22、S12S26和S19S27。
PCRs were performed on genomic DNAs of four Chinese white pear （Pyrus bretschneideri） cuhivars Donghuang, Xinliyihao, Hongpisu and Boshanehi using degenerate primers derived from conserved amino acids FTQQYQ and anti-IIWPNV of Japanese pear S1-8-RNases. Restricted digestion analysis of PCR products revealed that Xinliyihao and Donghuang had a common S8-allele. Sequencing and bioinformatics analysis of undigested PCR product showed that four alleles from the four eultivars were distincted from published S1-19-alleles at the DNA and deduced amino acid sequences level, thereby being identified as new alleles. They were named as S20-, S22-, S26- and S27-alleles, respectively （GenBank accession numbers AY250988, AY250990, AY339396 and AY339397）. Finally, the four eultivars were S-genotyped as follows ： Donghuang （S8S20）, Xinliyihao （S8S22）, Hongpisu （S12S26） and Boshanehi （S19S27）.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第4期859-864,共6页
Acta Horticulturae Sinica
基金
教育部重点项目
湖南省教育厅青年基金项目(101-4586)
国家林业局重点项目(2006-12)
湖南省自然科学基金项目(9347)
湖南省科技计划项目(5014)
中南林学院引进人才项目(101-0023)
