摘要
目的：研究枯草芽孢杆菌SN-02发酵液的抑菌谱及稳定性。方法：以28种植物病原菌为供试菌,杯碟法测定SN-02菌发酵液的抑菌谱;以烟草靶斑病菌为指示菌,杯碟法测定发酵液的热稳定性、酸碱稳定性及传代稳定性。结果：SN-02菌发酵液对28种供试菌株的抑菌圈直径在20mm以上。将发酵液于120℃处理2.5h,-20℃处理25d抑菌活性没有明显变化;发酵液在pH 4-9时抑菌活性无明显变化,在pH 1-3和pH 10条件下抑菌活性明显下降;连续培养10代,发酵液抑菌活性没有下降。结论：SN-02菌发酵液抑菌谱较广,耐高温和低温,传代稳定性好,但在强酸和强碱条件下稳定性较差。
Objective： Inhibitory spectrum and stability of Bacillus subtilis SN - 02 fermentation broth were studied. Methods： Cylinder - plate assay was used to mensurate inhibitory spectrum on 28 plant pathogens and to determine stability of the fermentation broth of Bacillus subtilis SN - 02 on Thanatephorus cumcumeris. Results：The inhibition diameters to 28 plant pathogens of the fermentation broth was up to 20mm.The fermentation broth could keep activity when treated at temperature 120℃ for 2.5h, - 20℃ for .25d and the pH value between 4 to 9, however, the inhibitory activity decreased obviously when pH value between 1 to 3 and 10. The inhibitory activity was not changed when the strain SN - 02 was subcultured for 10 generations. Conclusion： The inhibitory spectrum of strain SN- 02 fermentation broth was wide, stable to heat and low temperature, and good genetic stability, but the inhibitory activity fell down in extreme alkaline and extreme acid condition.
出处
《生物技术》
CAS
CSCD
2007年第4期71-73,共3页
Biotechnology
基金
辽宁省农业科技攻关招标项目资助(No.2004011)
关键词
枯草芽孢杆菌
发酵液
抑菌谱
稳定性
Bacillus subtilis
fermentation broth
antimicrobial spectrum
stability
