摘要
为解决油页岩在开采利用中有机物对地下水的污染问题,试验采用驯化培养的石油降解菌,对地下水中的有机污染物进行生物降解。在低温（10℃）、120r/min条件下,通过室内试验,确定菌群降解地下水中有机污染物的最佳pH为7,接种量为5mL（8×106个/mL）,初始浓度为200mg/L,辅助氮源为氯化铵（0.05%）,辅助磷源为磷酸二氢铵（0.05%）。在此最佳条件下,反应6天后总石油烃质量浓度为33.94mg/L,菌群对总石油烃及COD的降解率分别为83.03%及67.58%。通过对降解过程的动力学研究分析可知,总石油烃初始浓度为20mg/L时的降解符合一级动力学方程,50~300mg/L时的降解符合零级动力学方程。
To solve the problem of organic pollutants product in groundwater during exploitation and utilization of oil shale, the article took the oil degrading strains after domestication and cultivation to research the bio-degradation of the organic pollutants. The indoor research conducted at low temperature （10℃ ） and at shaking speed of 120 r/min. The experimental results show that： when the initial concentration of 200 mg/L, the optimum condition of the strains using the organic pollutants in groundwater was at pH 7, for inoculation of 5 mL, used ammonium chloride（0.05%） as the best nitrogen source and potassium sulfate（0.05%） as the best source of phosphorus. After 6 days at the optimum condition the concentration of total petroleum hydrocarbon could reduce from 200 mg/L to 33.94 mg/L. By the strains the degradation rate of the total petroleum hydrocarbon and COD was 83.03% and 67.58%, respectively. The biodegradation was proved to be first-order kinetic equation by kinetic analysis of degradation process under the initial concentration of the total petroleum hydrocarbon at 20 mg/L, and the total petroleum hydrocarbon initial concentration between 50-300 mg/L degradation meet zero-order kinetic equation.
出处
《中国农学通报》
CSCD
2013年第23期165-170,共6页
Chinese Agricultural Science Bulletin
基金
中央
国家其他部门项目"油页岩废水的高效生物处理研究"(450060481102)
中央
国家其他部门项目"页岩油微生物驱提技术研究"(OSR-03-06)
关键词
油页岩
地下水
有机污染物
生物降解
oil shale
groundwater
organic pollutants
bio-degradation
