摘要
目的:检测survivin在弥漫大B细胞淋巴瘤(DLBCL)中的表达,及其与细胞增殖和凋亡的相关性。方法:收集77例DLBCL和15例反应性增生淋巴组织,运用免疫组织化学EnVision法检测survivin和细胞增殖相关抗原Ki-67的表达,采用TdT介导的dUTP缺口末端标记技术(TUNEL)检测细胞凋亡指数。结果:Survivin在DLBCL中表达的阳性率为74%(57/77),在反应性增生淋巴组织中表达的阳性率为13%(2/15),二者差异有显著性(P<0.05)。Survivin表达与淋巴瘤增殖指数、凋亡指数呈明显正相关(r值分别为0.741,0.325,P<0.01)。结论:Survivin基因在DLBCL中表达上调,可能通过促进细胞增殖对DLBCL的发生发展起重要作用。
Objective To investigate gene expression of survivin and its relationships with cell apoptosis and proliferation in diffuse large B-cell lymphoma （DLBCL） tissues. Methods Immunohistochemistry methods and Tdt-mediated dUTP nick end labeling （TUNEL） methods was used to detect the expression of surviving, Ki-67 and cell apoptosis in DLBCL tissues （n=77） and reactive hyperplasia lymphoid tissues （n=lS）, respectively. Results The expression of survivin was significantly lower in reactive hyperplasia lymphoid tissues （13%, 2/15） than that in DLBCL tissues （74%, 57/77）（P 〈 0.05）, being positively correlated with proliferation index and apoptosis index （r=0.741, 0.325, P 〈 0.01 ）. Conclusion Survivin is highly expressed in DLBCL tissues, which may contribute to the progression of DLBCL by regulating cell proliferation.
出处
《实用医学杂志》
CAS
2007年第18期2856-2858,共3页
The Journal of Practical Medicine
