摘要
以5个毛白杨基因型(TC121、TC152T、C332、TC510和TC970)叶片为外植体,研究毛白杨不同基因型叶片再生能力的差异,通过单因子和正交试验设计,建立并优化毛白杨叶片再生体系.结果表明,毛白杨叶片离体再生能力受基因型的影响,基因型间的叶片再生率差异显著.其中基因型TC152再生率最高,在MS+6-BA 1.0 mg/L+NAA0.05 mg/L+蔗糖30 g/L+琼脂6 g/L培养基上可达到97.0%;基因型TC970在MS+6-BA 0.5 mg/L+NAA 0.05mg/L+蔗糖30 g/L+琼脂6 g/L培养基上再生率可达到94.7%;经培养基优化后,基因型TC332再生率提高至72.3%,而基因型TC510和TC121再生率仍低于50.0%.
The leaves of five genotypes（TC121,TC152,TC332,TC510 and TC970） in Populus tomentosa Carr.were used as explants to study the differences of in vitro regeneration competences among genotypes.Single factor and orthogonal experiment designs were used to establish and optimize the leaf regeneration system of P.tomentosa.The results showed that the regeneration competences were affected by genotypes,and there were obvious differences among genotypes in regeneration rates.The regeneration rate of TC152 reached 97.0% on the medium of MS ＋ 6-BA 1.0 mg/L＋ NAA 0.05 mg/L ＋ sucrose 30 g/L ＋ agar 6 g/L.The regeneration rate of TC970 was 94.7% on the medium of MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.05 mg/L ＋ sucrose 30 g/L ＋ agar 6 g/L.After medium optimization,the regeneration rate of TC332 had been improved to 72.3%.However,the regeneration rates of TC510 and TC121 remained below 50.0%.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2007年第5期38-43,共6页
Journal of Beijing Forestry University
基金
"十一五"国家科技支撑计划课题(2006BAD01A15)
关键词
毛白杨
基因型
再生
组织培养
Populus tomentosa Carr.,genotype,regeneration,tissue culture
