摘要
目的:研究血清基质金属蛋白酶-2(MMP-2)在肾纤维化进展过程中的变化,评价血液中MMP-2在肾脏纤维化诊断中的价值。方法:选择慢性肾小球肾炎患者33例,按照肾脏病理纤维化以及肾小球硬化程度分为增生组(18例)和纤维化组(15例),另设8例健康体检者为正常对照组。采用明胶酶谱法检测各组血清MMP-2酶原、活酶水平,比较各组血液中MMP-2水平。结果:与对照组比较,增生组MMP-2活酶水平明显升高(P<0.01),纤维化组MMP-2活酶水平则降低(P<0.05)。与增生组比较,纤维化组尿蛋白定量(U-pro)降低(P<0.01),血清纤维蛋白原(FIB)升高(P<0.05)、纤维连接蛋白(FN)升高(P<0.01),而血脂(CHO、TG)、血肌酐(Scr)水平差异无显著性。结论:血清MMP-2在慢性肾炎发展的不同时期呈现截然相反的水平变化,测定血清MMP-2水平有助于了解疾病的发展及判断预后。
Objective To study the change of matrix metalloproteinase-2 （MMP-2） in the development of renal fibrosis and evaluate the diagnostic value of blood MMP-2 level in renal fibrosis. Methods Thirty three patients with chronic glomerulonephritis were divided into proliferation group （n= 18） and fibrosis group （n= 15） according to renal fibrosis degree in renal pathology. Another 8 healthy subjects were used as control group. The levels of pro-MMP-2 and MMP-2 in serum were measured with the method of SDS-PAGE enzymography. Then the levels of pro-MMP-2 and MMP-2 in serum were compared between various groups. Results The serum MMP-2 level in proliferation group was much higher than that in control group （P〈0. 01） and it was lower in fibrosis group than that in control group （P〈0.05）. Compared with proliferation group, the content of urine protein in fibrosis group decreased obviously （P〈0. 01）, serum fibrinogen level increased markedly （P〈0. 05）, FN increased （P〈0. 01）; there were no obvious differences of plasma lipid and creatinine level （P〉0.05）. Conclusion There is a significant difference of serum MMP-2 between suffering group and normal control group. Therefore the detection of serum MMP-2 level can be conducive to understand the development of disease and estimate prognosis.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第4期741-743,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(200505130)
