摘要
目的观察酸性氧化电位水(EOW)对无菌室物体表面消毒后的效果。方法采用EOW浸泡拖布、抹布擦拭实验室、细胞培养室内的地面、墙、桌面,水浴锅内表面及拖鞋用EOW各浸泡5 min,前5处物体表面经擦拭、浸泡后,用培养皿扣贴取材,并按A、B、C、D 4组进行菌落计数;A组:为消毒前;B组:使用EOW擦拭、侵泡消毒后;C组:采用紫外线照射30 min后,从5处表面取材培养;D组:采用紫外照射30 min后,用EOW擦拭、浸泡后消毒采样培养。结果A组:GLP实验室、细胞培养室内环境表面均有菌落,有的无法计数;B组:除水浴锅中有1个平皿长出1个菌落处,有效率达90%;C组:虽经紫外线照射,空气中消毒效果较好,但从5处物体表面取材培养,可能与紫外线消毒离地面的距离较高有关;D组:细菌培养均为阴性。结论EOW消毒效果肯定、价格低廉,大面积、高处擦拭消毒使用方便,对皮肤刺激性小,符合环保要求,值得推广应用。
OBJECTIVE To observe the effect of electrolyzed oxidizing water （EOW） on the surface sterilization of indoor environment. METHODS Sterilized the surface of ground, wall, table, mob and thermostat-controlled water-bath in cell culture room and good laboratory practice （GLP） with EOW, then to draw the materials and culture with culture dish for 48 h in a 37℃ incubator, and count the colony number. Sterilization method and grouping： group A treated as a control, group B sterilized with EOW, group C sterilized with ultraviolate ray for 30 min and group D first treated with ultraviolate ray for 30 min, then sterilized with EOW. RESULTS In group A, the bacteria were overgrew and formed flakiness in 10/10 culture dishes; 1 colony was formed in group B, the sterilization effective rate was 90%. The bacteria culture of group C found no bacteria growing after sterilization with ultraviolate ray, however, sample from surface and culture after sterilization were seen bacteria, though the number of bacteria was less than group A. The bacteria culture outcome of group D was negative. CONCLUSIONS The EOW has a good sterilization effect, it is safe and untoxic, costly cheap and convenient to use, and fit to claim of environmental protection.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第8期969-970,共2页
Chinese Journal of Nosocomiology
关键词
酸化电位水
消毒
效果
Electrolyzed oxidizing water
Sterilization
Effects
