摘要
目的探讨125I粒子照射对CL187结肠癌细胞凋亡和周期的影响。方法采用人CL187结肠癌细胞系体外培养,实验分4组:空白对照组、2Gy照射组、5Gy照射组、10Gy照射组。照射组均选取9.25×104MBq活度的粒子,以2.77cGy/h的初始剂量率照射。照射结束后48h收集细胞,用膜联蛋白(annexin)和碘化丙啶(propidium iodide,PI)双染色法测量细胞凋亡,用碘化丙啶单染测量细胞周期。结果照射后48h,照射2、5、10Gy组细胞凋亡指数分别为13.74%±1.63%、46.27%±3.82%、32.58%±3.61%,显著高于空白对照组1.67%±0.19%(q=7.594,P<0.05;q=28.059,P<0.05;q=19.447,P<0.05)。空白组细胞G2/M期阻滞的比率26.44%±2.53%显著低于照射2、5、10Gy组34.61%±2.79%、59.84%±4.96%、42.59%±3.21%(q=4.039,P<0.05;q=16.513,P<0.05;q=7.985,P<0.05)。结论125I粒子持续性低剂量率照射主要通过凋亡杀伤CL187肿瘤细胞,引起凋亡的主要机制为G2/M期阻滞。
Objective To study the effects of 125^Iseeds irradiation on apoptosis and cell cycle of CL187 colon cancer cells. Methods In vitro cultured human CL187 colon cancer cells were randomly divided into 4 groups： Control group, Irradiation groups under 2, 5 and 10 Gy respectively, for which the activity of the seeds was determined at 9.25 × 10^4 MBq, and the initial dose to be 2. 77 cGy/h. All the cells were collected 48 hours after irradiation. The apoptosis were detected by using double staining method with annexin and propidium iodide （PI） , and the cell cycle by using PI staining method. Results The apoptosis rates were 13.74% ± 1.63% , 46. 27% ± 3.82% , 32.58% ± 3.61% respectively in 2 Gy, 5 Gy, and 10Gy groups, significantly higher than that of control group （ 1.67% ± 0.19% , q = 7. 594, P 〈 0.05 ; q = 28. 059, P 〈 0.05 ; q = 19. 447, P 〈 0.05 ）. The G2/M cell cycle arrest in control group （26.44% ± 2.53% ） was significantly lower than those in 2 Gy, 5 Gy, and 10 Gy Irradiation groups （34.61% ± 2.79%,59.84% ±4.96%,42.59% ±3.21%; q =4.039, P 〈0.05; q = 16.513, P 〈0.05; q =7.985, P 〈0.05）. Conclusions Continuous exposure to low dose irradiation of 125^I seeds kills CL187 colon cancer cells primarily by apoptosis, of which the major mechanism is G2/M cell cycle arrest.
出处
《中国微创外科杂志》
CSCD
2007年第8期804-807,共4页
Chinese Journal of Minimally Invasive Surgery
