摘要
建立了用反相高效液相色谱法同时测定化妆品中9种禁用四环素类抗生素。采用甲醇超声提取，Kromasil C18柱（250 mm×4.6 mm i.d.，5μm）分离测定。9种被测物在12 min内均得到良好的分离。在1-100μg/mL范围内均与其各自对应的峰面积呈良好线性关系（r≥0.9991），回收率在85.5%-105.7%之间，精密度RSD〈3.2%，该方法的检出限（S/N=3）为0.05-0.5μg/mL。该法可用于祛痘类化妆品中9种禁用四环素类抗生素的检测。
A method for the determination of 9 tetracycline antibiotics （TCs） by reversed-phase high performance liquid chromatography is described. TCs were successfully separated on a Kromasil Cls column （250 mm × 4.6 mm i.d., 5 μm）. The calibration curves of 9 tetracycline antibiotics were linear （r≥0.9991） between 1 and 100μg/mL. The limit of detection was between 0.05 μg/mL and 5.0 μg/mL. The average recoveries were between 85.5% and 105.7% with RSD （ n = 6） values between 0.1% and 3.2%. The results showed that the method was sensitive, accurate, convenient and quick, which can be used for simultaneous detection of the 9 tetracyclines residues in cosmetics.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第8期52-55,共4页
Chinese Journal of Analysis Laboratory
关键词
高效液相色谱法
化妆品
四环素
High performance liquid chromatography
Cosmetics
Tetracycline
