摘要
目的:观察IFN-γ和TNF-α对正常精子顶体酶活性和顶体反应率的影响,并对其变化机制进行初步探讨。方法:36例精液常规分析基本正常标本,经75%Percoll分离后,分别或联合使用IFN-γ和TNF-α处理(终浓度均为30ng/ml)。采用BAEE/ADH联合法测定精子顶体酶活力的变化。用三色染色法观察精子顶体反应率的变化。用高效液相色谱法(HPLC)检测精子NO含量。用试剂盒法测定Na+-K+-ATPase、Ca2+-ATPase和SOD活性。结果:IFN-γ和TNF-α单独及联合使用时,均可显著降低精子顶体酶活性和顶体反应率(P<0.05或P<0.01),且TNF-α的抑制作用更强些;IFN-γ可使精子Na+-K+-ATPase、Ca2+-ATPase和SOD活性显著降低(P<0.01),且两者协同作用更低,但TNF-α对Na+-K+-ATPase和Ca2+-ATPase活性几乎无作用;IFN-γ、TNF-α及两者合用时均使精子NO含量显著增加(P<0.01)。结论:IFN-γ和TNF-α对精子顶体酶活性及顶体反应率有一定的抑制作用,并且可能通过对精子Na+-K+-ATPase、Ca2+-ATPase、SOD活性以及NO含量等多方面影响来实现的。
Objective： To investigate the effects of interferon-gamma （IFN-γ） and tumor necrosis factor alpha（TNF-α） on the sperm acrosin activity and the rate of acrosome reaction and to probe into their mechanisms. Methods ： Thirty-six nearly normal semen sampies were treated with IFN-γ and/or TNF-α after isolated by 75% Percoll. The sperm acrosin activity was tested by the method of BAEE/ADH Unity, the rate of acrosome reaction observed by Triple-stain technique, the NO concentration measured by HPLC and the activities of Na^＋ -K^＋ -ATPase, Ca^2＋ -ATPase and SOD assayed by kit method. Results ： Both IFN-γ and TNF-α could decrease sperm acrosin activity and acrosome reaction （P 〈0.05 or P 〈0.01 ）. TNF-α showed stronger inhibiting effect, IFN-γ markedly reduced the activities of Na^＋ -K^＋ -ATPase, Ca^2＋ -ATPase and SOD in sperm （P 〈 0.01 ）, and their synergistic action was weaker. However TNF-α produced hardly any effect on Na^＋ -K^＋ -ATPase and Ca^2＋ -ATPase. The NO concentration in sperm was significantly increased by IFN-γ and/or TNF-α （ P 〈 0.01 ）. Conclusion ： IFN- γ and TNF-α have some inhibiting effect on sperm acrosin activity and the rate of acrosome reaction, which could be attributed to their influence on the activities of Na^＋ -K^＋ -ATPase, Ca^2＋ -ATPase and SOD, the NO concentration and so on.
出处
《中华男科学杂志》
CAS
CSCD
2007年第8期681-684,共4页
National Journal of Andrology
基金
黑龙江省卫生厅课题(2005-006)
关键词
干扰素-Γ
肿瘤坏死因子
精子
受精能力
interferon-gamma
tumor necrosis factor-alpha
sperm
fertilizing capacity
