摘要
目的研究粘着斑激酶磷酸化与大鼠血管内膜剥脱术后血管重塑的关系。方法通过建立大鼠主动脉内皮剥脱术后再狭窄模型,将雄性大鼠随机分为5组,非内皮剥脱对照组(CN)、内皮剥脱术后4、8、16和24d组。取内膜剥脱部位血管段进行形态学观察,并用Western blolt方法检测FAK总含量及磷酸化型FAK的含量,最后密度扫描分析。结果CN组可检测到少量FAK,主要是以非磷酸化形式存在;4d组FAK和磷酸化FAK含量均明显升高(37%、68%,vesCN组,P<0.05),随着损伤时间的延长,FAK和磷酸化型FAK含量显示相同的变化趋势。但FAK在术后第8天达到峰值,一直持续到术后第16天,此后开始下降;磷酸化型FAK峰值出现在术后16d,在峰值水平上持续时间较短。形态学观察显示,术后8d可见新生内膜中局灶性细胞增殖,术后16～24d内膜呈弥漫性增厚。结论FAK参与大鼠血管内膜剥脱术后血管重塑,血管新生内膜中细胞增生的程度与磷酸化型FAK及FAK总含量的变化密切相关。
[Objective] To study the role of focal adhesion kinase （FAK） phosphorylation in vascular remodeling after balloon endothelial denudation in rats. [Methods] The rat models of balloon endothelial denudation were built. Male Wistar rats were randomized into five groups. No balloon endothelial denudation control group and 4 day, 8 day, 16 day and 24 day model group after balloon endothelial denudation. Western blot was used to detect the expression and activity of focal adhesion kinase （FAK）. hyperplasia of intima was observed. [Results] Compared with the control group, the expression and activity of focal adhesion kinase （FAK） in model group rapidly increased at day 4 after balloon injury, the expression of FAK reached its peak at day 8, and began to decrease at day 16. Focal hyperplasia of intima was found after 8 day after balloon endothelial denudation, diffuse hyperplasia of intima were found after 16～24 day after balloon endothelial denudation. [Conclusion] FAK takes part in vascular remodeling after balloon endothelial denudation in rat. The level of intima hyperplasia is positively associated with the lever of focal adhesion kinase （FAK） and focal adhesion kinase phosphorylation.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第15期1844-1846,共3页
China Journal of Modern Medicine
基金
湖北省科技厅面上项目资助(2005ABA086)
关键词
粘着斑激酶
内皮剥脱术
血管重塑
血管再狭窄
focal adhesion kinase （FAK）
balloon endothelial denudation
vascular remodelling
