摘要
木薯是广西的主要经济作物之一,主要用于生产淀粉,其副产物木薯渣中仍残留大量淀粉,弃之会造成浪费和环境污染。本研究旨在探索一条利用木薯渣的新途径。以木薯渣为原料,利用工业糖化酶和产朊假丝酵母(Candidautilis)固态发酵,制取单细胞蛋白饲料。结果表明:通过高压灭菌预处理物料效果较好;氮源的添加量分别为:尿素3%,硫酸铵6%;通过单因素正交实验得最佳工艺条件为:糖化酶用量为4%,含水量为70%,发酵时间72h,酵母接种量10%。粗蛋白质含量由底物的11.59%提高到产物的28.28%,提高了16.69个百分点。与其它方法比较,本方法利用工业糖化酶,发酵过程易于控制,不需要选育淀粉分解菌,使培养成本降低,具有一定的工业应用潜力。
In Guangxi province of China, the cassava is one of the major economic crops, and is mainly used to produce starch. During which, massive cassava residues will be produced simultaneously, and if it is abandoned, it will cause the waste of resource and environment pollution. A means of utilizing the cassava residues to produce single-ceU protein feed via solid-state fermentation with industrial saccharidase and Candida utilis was proposed and studied. The experimental results show that the most suitable pretreatment of the cassava residues used as raw material for solid-state fermentation is the high temperature sterilization, and the adequate concentrations of urea and sulfuric ammonium in culture medium are 3% and 6%, respectively. Through single factor orthogonal experiment, the optimal fermentation conditions were obtained as follows. The saccharidase concentration used is 4%, moisture content is 70%, fermentation time is 72 h and inoculum concentration is 10%. Under those conditions, the crude protein content increases from 11.59% of the substrate before fermentation to 28.28% of the fermentation product. Comparing with other cassava residues utilizing methods, the method proposed has the advantages of easy control and no need of the starch decomposing eumycete, and these advantages will cause the method proposed has lower cultivation cost and potential availability for industrial production.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2007年第4期720-724,共5页
Journal of Chemical Engineering of Chinese Universities
基金
广西南宁科技基金项目
关键词
木薯渣
固态发酵
单细胞蛋白
产朊假丝酵母
工业糖化酶
cassava residues
solid-state fermentation
single-cell protein
candida utilis
industrial saccharidase
