摘要
在含固体残渣 8%的酒精废液中 ,加入尿素 2g/L、玉米浆 2mL/L ,经接种白曲霉基因工程菌TR1 2菌株 ,在 32℃摇床培养 70h后 ,可获得含糖化酶 2 4 6U/mL和耐酸性α 淀粉酶 1 3 42U/mL的转化液。转化液直接循环利用于无蒸煮酒精发酵配料工序 ,不仅不影响酒精生产的产量和质量 ,而且能有效地减轻环境污染压力 ,节约水资源 。
Five grams of urea and two milliliters of corn syrup were added into 1 liter ethanoldistilled wastewater with 8% solid dregs The activity of acid resistant α amylase and glucoamylase produced by inoculated Aspergillus kawachii genetic engineering strain TR12 in the transformed liquid reached a level of 13 42U/mL and 246U/mL respectively after fermentation at 32℃ by the shaking flask for 70 hours The transformed liquid was circularly applied to the ethanol ingredient process without cooking room, not only it didn't influence the ethanol output and quality, but also it could efficiently reduce the pollution of ethanol distilled wastewater and the cost of ethanol production
出处
《微生物学通报》
CAS
CSCD
北大核心
2004年第4期26-29,共4页
Microbiology China
基金
教育部留学回国人员启动基金 (No 2 0 0 0 3 67)
关键词
酒精废液
微生物转化
转化液回用
The ethanol distilled wastewater,Transformed by microorganism,Re use of the transformed liquid
