摘要
目的对比观察异丙酚和咪达唑仑对大鼠肝脏缺氧复氧损伤的影响。方法24只健康成年Wistar大鼠随机分为4组:对照组(A组)、缺氧复氧组(B组)及咪唑安定和异丙酚预处理组(C组和D组),每组6只。采用酶联免疫吸附法检测大鼠肝组织8-异前列腺素F2α(8-iso-PGF2α)水平,硝酸还原法测定其一氧化氮合酶(NOS)活力和NO含量。结果缺氧复氧损伤后,B组肝组织中8-iso-PGF2α水平明显升高,而NOS活力和NO含量则明显降低,与A组比较差异有显著性(P<0·05);C、D两组与B组相比,肝组织中8-iso-PGF2α水平明显下降,而NOS活力和NO含量明显上升,差异有显著性(P<0·05);D组的肝组织8-iso-PGF2α含量明显低于C组,而其NOS活力和NO含量明显高于C组(P<0·05)。结论咪达唑仑和异丙酚均能对大鼠肝脏缺氧复氧损伤起到明显的保护作用,但咪达唑仑的保护效应不如异丙酚。
Objective To investigate the preventive effects of midazolam and propofol on liver against hypoxia/reoxgenation （H/R） injury in rats. Methods Twenty-four Wistar rats of both sexes weighing 200-250g were randomized into four groups： control group （group A）, hypoxia/reoxygenation （group B）, H/R ＋ midazolam （group C） and H/R ＋ propofol （group D）. The 8-iso-prostaglandin F2α （8-iso-PGF2α） levels in the hepatic tissue were determined by enzyme-linked immunosorbent assay （ELISA）. The contents of nitric oxide （NO） and the activity of nitric-oxide synthase （NOS） in hepatic tissue were determined by biochemistry methods. Results After hypoxia and reoxgenation injury in rats, the 8-iso-PGF2α level of hepatic tissue in group B was significantly higher than that in group A （ P〈 0. 05）, and the contents of NO and the activity of N（3S were significantly lower than those in group A （P〈0. 01）. The levels of 8-iso-PGF2α in liver tissue in groups C and D decreased significantly and were lower than that in group B （P〈0. 05）. The contents of NO and the activity of NOS in groups C and D increased significantly and were higher than those of group B （P〈0. 01） But the level of 8-iso- PGF2α in group D was significantly lower than that of group C. The contents of NO and the activity of NOS of group D were higher than those of group C （P〈0. 05）. Conclusions Both midazolam and propofol can prevent liver against H/R injury in rats. But the preventive effect of midazolam is weaker than that by propofol.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第7期732-733,共2页
Medical Journal of Chinese People's Liberation Army
