摘要
采用HPLC法测定甲基多巴中3-甲氧基甲基多巴及其它有关物质的含量,并与药典中的TLC法进行对比.色谱条件为：色谱柱：phenomenex LUNA C18分析柱（4.6 mm×250 mm, 5 um）,流动相：乙腈-2.0%磷酸氢二钠水溶液,加入磷酸调节pH至5.0（10∶90）,流速：1 mL/min,检测波长：280 nm,进样量：20 μL.该方法中甲基多巴和3-甲氧基甲基多巴及氨基丙腈分离度良好,线性范围分别为0.002～0.1 μg（r=0.999 97）,检测限：1 ng;0.008～0.2 μg（r=0.999 8）,检测限：2 ng.本法简单、专属,能有效快速地控制产品质量.
A HPLC method was established to determin the concentrations of 3-methoxymethyldopa and the related substances in methyldopa. A Phenomenex LUNA C18 column （4.6 mm×250 mm, 5 um） was used. The mobile phase was acetonitrile-2.0% dihydrophosphate sodium which was adjusted to pH 5. 0（10 ： 90）. The flow rate was 1 mL·min^-1 , the detection wavelength was 280 nm, and the injection volumn was 20 μL. Using the above method, methyldopa, 3-methoxymethyldopa and benzenerproanenitrile were separated very well. The linear respons range were 0. 008 - 0. 2 μg（r=0. 999 8） for 3-methoxymethyldopa and 0.002-0. 1 μg（r= 0. 999 97）for methyldopa, respectively. The detected limits were 2 ng for 3-methoxymethyldopa and 1 ng for methyldopa, respectively.. The method is simple, selective, accurate and quick for quality control.
出处
《浙江工业大学学报》
CAS
2007年第4期404-407,共4页
Journal of Zhejiang University of Technology
