摘要
选用双鳞片法切割红花石蒜鳞茎为外植体，采用MS基本培养基，以两种植物生长调节剂NAA和6-BA不同浓度配伍，研究了不同消毒时间和不同激素浓度对小植株诱导和不定芽诱导的影响。结果表明：利用0．1％升汞溶液浸泡石蒜鳞茎10～15min消毒效果最好；MS＋2mg／L6-BA＋0．2mg／L NAA是小植株诱导的最佳培养基；MS＋NAA2mg／L＋6-BA2mg／L是不定芽诱导的最佳培养基；MS＋2mg／L NAA对于小鳞茎的生根效果最好。
The bulbs of Lycoris radiata were selected as explants with double bulb - scale method, using MS as basic culture medium and mixing two plant growth regulators NAA and 6 - BA with different concentrations. The effects of different sterilization time and different hormone concentrations on the vegetable induction and adventitious buds induction were studied. The results showed as follows： being dipped in 0.1% Hg solution for 10 - 15 minutes had the best effect on bulb culture of Lycoris radiata; MS ＋2mg/L 6 - BA ＋0.2 mg/L NAA was optimal for vegetable induction; MS ＋ NAA 2mg/L ＋6 - BA 2mg/L was better for adventitious buds induction, and MS ＋2 mg/L NAA had the best effect on the rooting of bulb.
出处
《江西农业学报》
CAS
2007年第7期57-59,共3页
Acta Agriculturae Jiangxi
基金
湖北省教育厅科技攻关计划项目(2002P1004)
湖北省科技创新团队计划(鄂教科[2003]7号)
关键词
红花石蒜
鳞茎
组织培养
不定芽
生根
Lycoris radiata Herb
Bulb
Tissue culture
Adventitious bud
Rootage
