摘要
将人工合成的哺乳动物型GnRH六聚体基因插入原核表达质粒pMAL-c2中,重组质粒转化到大肠埃希菌BL21中,采用IPTG进行诱导表达,用多糖树脂亲和层析法纯化蛋白,用SDS-PAGE对纯化蛋白进行分析,用间接ELISA方法鉴定融合蛋白的反应原性。结果表明,与麦芽糖结合蛋白基因融合的Gn-RH六聚体基因在大肠埃希菌BL21中能够高效表达;用多糖树脂进行亲和层析纯化蛋白,具有良好的纯化效果;表达的融合蛋白分子质量大小与理论值一致,且融合蛋白与GnRH抗体的反应较好。
The synthetic mammalian GnRH-6 gene was inserted into the expression plasmid pMAL-c2. The recombinant plasmid was transformed into E. coli BL21, where the expression of GnRH-6 gene was induced with IPTG. Amylose resin was used to purify the protein by the affinity chromatograph method. The purified products were analyzed using the SDS-PAGE. The reaetiongenieity of fusion protein was identified by indirect ELISA. The results showed that the GnRH-6 gene in fusion with the gene encoding maltose binding protein could be expressed at a high level. The fusion protein could be purified efficiently by affinity chromatograph method. The molecular weight of the fusion protein was equal to that of the theory. the reaetiongenieity of fusion protein with GnRH antibody was very high.
出处
《动物医学进展》
CSCD
2007年第7期12-16,共5页
Progress In Veterinary Medicine
基金
安徽省教育厅自然科学研究项目(KJ2007B175)
