摘要
采用离体灌流孵育技术和促性腺激素的放射免疫测定方法 ,对长臀 (Cranoglanisbouderius)脑垂体碎片促性腺激素的分泌进行了研究。结果表明 :持续的促性腺激素释放激素类似物 (GnRH A)能显著刺激退化期的长臀离体脑垂体碎片促性腺激素 (GTH)的分泌 ,并且长臀脑垂体碎片对持续的GnRH A刺激未表现出脱敏性 ,该结果与胡子鲇和鲇鱼相似 ,而与金鱼和鲤科鱼类不同 ;重复脉冲GnRH A刺激对长臀脑垂体碎片GTH分泌具有促进作用 ,而且存在剂量依存关系 ,与鲇鱼和鲤科鱼类相类似。上述结果表明在长臀的人工繁殖中可以用持续高浓度GnRH A刺激对长臀进行催熟和催产。
The gonadotropin (GTH) secretion from pituitary fragments of helmet catfish (Cranoglanis bouderius) was studied by a perfusion incubation system and GTH radiommunoassay (RIA).The results showed that chronic administration of gonadotropin hormone-releasing hormone analogs (GnRH-A) was able to stimulate GTH release from pituitary fragments in vitro,which was consistent to African catfish (Clarias gariepinus) and catfish (Silurus asotus).The insensitivity to chronic administration of GnRH-A was not be observed in the helmet catfish,which was consistent to catfish and African catfish,but different from that in goldfish (Carassius auratus) and Cyprinus carpio haematopters.Repetitive pulse administration of GnRH-A was also able to stimulate GTH release from the pituitary fragments in dose-dependent manner in helmet catfish.These results showed that chronic administration of GnRH-A could have an effect on artificial propagation of helmet catfish.
基金
国家自然科学基金资助项目 ( 3 9970 5 86)
广东海洋渔业局淡水鱼类繁育中心资助项目
