摘要
采用叔胺化反应与季铵化反应两步高分子反应过程,制备了季铵化的聚乙烯亚胺(QPEI);以大肠杆菌(E.coli)为致病菌体,重点研究了高分子季铵盐QPEI的抗菌活性;采用平板活菌记数法考察了季铵化度及pH值等因素对其抗菌性能的影响规律;并采用β-半乳糖苷酶活性测定法,研究了QPEI的抗菌机理.研究结果表明,QPEI具有很强的抗菌能力,对浓度为109CFU/mL的菌悬液,在药剂量为15mg/L、接触时间为4min的条件下,杀菌率可达100%;QPEI的季铵化度对其抗菌性能影响很大,季铵化度越高,抗菌性能越好;在一定的pH值范围内,pH值越高,抗菌性能越好.β-半乳糖苷酶活性测定结果表明,QPEI的抗菌机理是基于杀菌过程,而不只是抑菌作用.
Quaternary polyethyleneimine （QPEI） was prepared via two procedures of macromolecnlar reactions, tertiary amination and quaterisation, and the antimicrobial properties of this polymeric quaternary ammonium salt were mainly studied by using Escherichia Coli as a disease-leading bacterium. The effects of quaternary degree and pH value on the antimicrobial properties were examined with the method of plate counting living bacterium, and the antimicrobial mechanism of QPEI was studied more deeply via measuring the activities of β-D-galactosidase. The experimental results indicates that QPEI possesses very strong antimicrobial ability, and the antimicrobial ratio can reach 100% when QPEI is contacting with a bacterium suspension of 109 CFU/mL under the conditions of a contacting time of 4 rain and a dose of 15 mg/L.The cationic degree of QPEI influences its antimicrobial properties to a great extent, and the higher the cationic degree is, the stronger the antimicrobial activity. In a certain region of pH values, the higher the pH, the stronger the antimicrobial ability is. The results of measuring activities of the enzyme of β-D-galactosidase shows that the antimicrobial mechanism of QPEI is based on a sterilizing bacterium process, and is not only based a restraining action.
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
2007年第7期627-632,共6页
Acta Polymerica Sinica
基金
山西省工业攻关项目(项目号051208)
关键词
高分子抗菌剂
聚乙烯亚胺
季铵盐
抗菌性能
抗菌机理
Polymeric antimicrobial, Polyethyleneimine, Quaternary ammonium salt, Antimicrobial property, Antimicrobial mechanism
