摘要
目的:观察刺梨提取物(CL)的抗肿瘤作用并探讨其机制。方法:应用艾氏腹水癌(EAC)小鼠模型观察CL的体内抗肿瘤作用。通过MTT法和台盼蓝拒染法绘制细胞生长曲线研究CL抗人子宫内膜腺癌(JEC)作用。用全自动生化分析仪测定细胞培养上清液中乳酸脱氢酶(LDH)含量,测定NBT还原能力以了解CL对肿瘤细胞的诱导分化作用。通过AO/EB荧光染色法和流式细胞仪检测,分析CL对肿瘤细胞凋亡的影响。采用流式细胞仪检测CL对肿瘤细胞增殖周期的影响。结果:与阴性对照组比,CL 10μg.mL-1化疗组EAC小鼠的寿命延长(P<0.05),胸腺指数和脾指数明显增大(P<0.05);CL对JEC细胞的抑制作用随CL剂量的增加和作用时间的延长而增强,CL10μg.mL-1作用JEC细胞96 h的IC50为0.05μg.mL-1;JEC细胞的生长曲线随着CL剂量的增加而逐渐下移;随CL剂量的增加,NBT阳性细胞百分率逐渐增加,细胞培养上清中LDH含量逐渐降低。CL对JEC细胞凋亡的诱导作用呈现剂量依赖性,CL 10μg.mL-1作用JEC细胞24 h的凋亡细胞百分率为25.59%(P<0.05)。CL作用24 h后,阻滞JEC细胞于G2-M期(P<0.05)。结论:CL具有一定的体内外抗肿瘤作用。提高机体免疫功能可能是CL体内抗肿瘤的机制之一;CL体外抗JEC细胞的机制与CL的细胞毒作用、诱导细胞凋亡和抑制G2-M期细胞分裂有关。
Objective： To investigate anticancer effects and potential mechanisms of CL, extract of Rosa roxburghii. Method： In vitro anticancer effect was observed in Ehrlich＇ s ascites carcinoma （EAC） mice model. Cell toxicity of CL on human endometrial adenocarcinoma cell line JEC （JEC） cells was measured by MTY reduction test and growth curves drawing by trypan blue dye exclusion method. Lactate debydrogenase （LDH） concentration of cultured medium was detected by auto-biocbemistry-meter. Cell differentiation was showed by detection of NBT reduction ability. Apoptosis was showed by AO/EB fluorescent staining and flow cytometer detection. Cell proliferation cycle was detected by flow cytometer. Result： Comparing with the negative group, life span of EAC mice treated with CL was prolonged （ P 〈 0. 05 ）, and thymus index and spleen index of them were raised （ P 〈 0. 05 ）. The inhibitory effect of CL on JEC cells was in concentration-and time-dependent manner. IC50 of CL on JEC cells was 0. 05 μg·mL^-1 in 96 hours. Growth curves showed right-shift with CL concentration increasing. The number of NBT positive JEC cells increased and the LDH concentration of cultured medium declined with CL increasing. Apoptosis of JEC cells with CL treated was induced in concentration-dependent manner, apoptotic percentage of CL 10μg·mL^-1 on JEC cells was 25.59% in 24 hours. CL arrested JEC cells in G2-M phase （P 〈0.05）. Conclusion： CL has certainly anticancer effects in vivo and in vitro. Anticancer effect of CL in vivo was in relation to enhancing immune function of EAC mice; anticancer mechanisms of CL on JEC cells may be its direct cytotoxic effect, inducing cell apoptosis and inhibiting cell segmentation.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第14期1453-1457,共5页
China Journal of Chinese Materia Medica
基金
贵州省科技基金资助项目(黔科字3050)
关键词
刺梨提取物
抗肿瘤
艾氏腹水癌
人子宫内膜腺癌
Rosa roxburghii
aticancer
Ehrlich＇s ascites carcinoma
human endometrial ad
