摘要
目的使用小鼠验证这样一个假设:外界病毒浸入诱发心肌炎时,机体的干细胞将进入心脏提高心肌的抗病毒能力。方法雄性BALB/c小鼠分为三组:小鼠胚胎干细胞对照组(ES),心肌炎病毒组(EM CV)及EM-CV加ES治疗组。通过尾静脉注射,令小鼠立即感染病毒。小鼠死亡率,炎性细胞浸润及心肌坏死等为观察指征。干细胞的游走及分化等通过免疫荧光法来验证。结果给予干细胞后的小鼠的存活率明显高于生理盐水对照组,炎性细胞侵润及心肌坏死亦明显低于生理盐水对照组。免疫荧光法表明,干细胞进入心肌并分化成新的心肌细胞。结论干细胞能明显提高心肌炎小鼠的存活率,减少心肌组织的坏死。同时,亦证明当心脏遭受病毒的侵入后,干细胞通过某种机理修复或再生心肌细胞,从而提高组织的抗病毒能力。
Objective We used mice to test our hypothesis that in response to viral invasion, stem cells may migrate into the heart and attenuate the effects of viral myocarditis. Methods BALB/c mice were divided into three groups, mouse embryonic stern cell （ES） control, encephalomyocarditis virus （EMCV）, and EMCV ＋ ES. Mice were immediately inoculated （ip） with EMCV after tail vein injection of ES. Animals were sacrificed at different days after EMCV inoculation. Mortality was recorded. Inflammatory cell infiltration and necrosis were evaluated by hematoxylin-eosin staining, ES migration and differentiation were identified by immunoflurescence. Results The mortality in the EMCV ＋ ES group （20%） was significantly decreased （p〈0. 05） compared to EMCV-alone group （36%）. The infiltration of inflammatory cells and myocardial necrosis was lower in the EMCV ＋ ES treated mice, too. Furthermore, the green fluorescent protein （GFP） and a-actinin data indicated that ES migrated into the heart and differentiated into myocytes after virus inoculation. In conclusion, ES significantly increased the survival of viral myocarditis mice and also decreased the necrosis and infiltration of inflammatory ceils, conclusion These results demonstrated the ability of stem ceils to mitigate the effects of viral infection on the heart.
出处
《佛山科学技术学院学报（自然科学版）》
CAS
2007年第3期33-37,共5页
Journal of Foshan University(Natural Science Edition)
关键词
心肌炎
小鼠胚胎干细胞
小鼠
心肌炎病毒
myocarditis
embryonic stem ceils, mouse, encepholomyocarditis virus
