摘要
【目的】对No.24菌株及其诱变株Ms-24菌株的原生质体的制备、融合、再生条件及融合子的生物活性进行了研究,探索了秦岭霉素的产量提高与融合条件的关系。【方法】采用荧光染色技术对融合子进行了标记,秦岭霉素含量的测定采用了HPLC法进行。【结果】融合后得到的3株高产秦岭霉素的融合子即为pf77,pf126和pf138菌株,其发酵产物的杀菌活性比原始No.24菌株分别提高了43.48%,60.87%和65.22%。HPLC检测发现,融合子pf77,pf126,pf138菌株发酵产物中秦岭霉素的含量与No.24菌株相比分别提高了36.59%,74.39%和91.46%。【结论】进行原生质体再生时,添加甘氨酸、溶菌酶的浓度要适中;原生质体融合时,酶解时间、聚乙二醇(PEG)的浓度和分子量也要适宜。
[ Objective ] Research was done to discuss the condition of preparation, fusion, regeneration of No.24 and mutagensis Ms-24 strain＇s protoplast,mensurate the bioactivity of fusants, and group the relationship between Qinlingmycin production and fusion condition. [Method] The fusants were marked with fluorescence, and the HPLC method was adopted to mensurate the content of Qinlingmycin. [ Result] Three high qinlingmycin-producing strain pf77,pf126 and pf138 was obtained. Bioassay results showed that the fungicidal activity of the three fusants increased by 43.48%, 60.87% and 65.22%, and the production of qinlingmycin increased by 36.59%, 74.39% and 91.46%. [Conclusion] Concentration of Glycine and lysozyme and treating time of protoplast, concentration of PEG and molecular weight should be Optimized in protoplast regeneration.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第7期1416-1421,共6页
Scientia Agricultura Sinica
基金
国家"863"高技术计划项目(2002AA245121)
"973"计划项目(2003CB114404)
