摘要
目的:研究IL-2Rα模拟肽CP对小鼠皮肤移植排斥反应的影响。方法:采用小鼠同种皮肤移植模型,通过淋巴细胞增殖试验、单向混合淋巴细胞反应、脾脏T淋巴细胞亚群分析和细胞因子分泌水平测定、皮肤移植物组织学改变、记录皮肤移植物平均存活时间(MST)研究环七肽CP的免疫抑制效应。结果:环七肽CP可降低小鼠脾细胞增殖反应的强度、减少受鼠脾脏T淋巴细胞中CD4+T淋巴细胞的数目并降低CD4+/CD8+的比值、下调受鼠脾脏T淋巴细胞诱导上清中IL-2和IFN的水平,并可延长皮肤移植物平均存活时间(MST);环七肽CP与免疫抑制剂CsA联用,显示二者具有协同效应。结论:环肽CP以IL-2Rα模拟肽的方式发挥生物学效应,可有效抑制小鼠皮肤移植排斥反应。
Objective ：To study the effects of IL-2Rα mimic peptide（CP） on skin allograft rejection in mice. Methods：Mouse skin allograft model was employed in all groups. The bioactivities were determined by lymphocyte proliferation, one-way MLR, assay of T lymphocyte subset and the level of cytokine in splenic cell culture supernatant. Results： CP inhibited lymphocyte proliferation ; decreased the numher of CD4^ ＋ T lymphocytes in spleen and the value of CD4^＋/CD^8＋ ; down-regulated the level of IL-2 and IFN in splenic cell culture supernatant, as well as prolonged the mean survival times（MST） of skin allografts. The combination of CP and CsA showed cooperativities. Conclusion：CP,as the IL-2Rα mimic peptide has the suppressive activities,which can efficiently inhibit skin allograft rejection in mice.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2007年第6期513-517,共5页
Chinese Journal of Immunology
