摘要
目的 研究Smad3基因剔除小鼠的体液免疫和细胞免疫。方法 采用流式细胞仪对其细胞免疫和体液免疫进行检测 ;并且应用ELISA方法对IgG抗体的吸光度进行测定。结果 纯合型 (- - )小鼠CD8+ 、CD4 + CD8+ 、IgG雌雄之间差异有显著性 ;CD4 + 、CD8+ 、CD3+ 、CD19+ 、IgG的值低于野生型 ;结论 CD4 + CD8+ 的比值同野生型比较属于异常 (与人的范围比较 )。因此 。
Objective The cellular immunity and humoral immunity of the mice with gene knock out Smad3 were studied. Methods The parameters of CD4 +,CD3 +,CD8 +,CD19 +,IgG were detected by FCM and ELISA.Results The parameters of CD8 +,CD4 +/CD8 +,IgG of homozygous mice were significant differences between genders. But the parameters of the CD4 +,CD8 +,CD3 +,CD19 +,IgG were lower than those of wild types. Conclusions It is reference value in using the mice lacking Smad3 for the study of disease of human immunity.
出处
《中国实验动物学报》
CAS
CSCD
2002年第3期148-150,共3页
Acta Laboratorium Animalis Scientia Sinica
基金
北京市科技项目专项基金 (95 49810 0 0 )
