摘要
目的:建立HPLC测定维生素C葡萄糖注射液中维生素C的含量。方法:采用NH2柱;以磷酸二氢钾溶液(取磷酸二氢钾2.72g,加水溶解并稀释成1000ml,用0.45μm微孔滤膜滤过即得)-乙腈(1∶1)为流动相;流速1.0ml/min;检测波长为245nm。结果:维生素C的浓度在5～26μg/ml范围内,呈良好的线性关系,相关系数为0.9998。平均回收率为99.71%,RSD=0.92%(n=9)。结论:本方法简便,准确、专属性强、重现性好,与国家标准结果一致。
Objective ：To establish an external standard HPLC method tor cletermmatlon of Vitamln C. Method： The liquid chromatography was carried out using a NH2 column（5μm, 4.6mm × 250mm）with detection wavelength at 245nm. The mobile phase as follows ：Acetonitrile-potassium phosphate monobasic（Dissolve 2.72g of disodium hydrogen phosphate in 1000mL of water） = 1 ：1.The flow rate was 1ml/min. Results：The calibration curve was linear in the range of 5-26μg/ml ,r=0. 9998. Average recovery（n= 9）was 99.71 % ,RSD= 0. 92 %. Conclusion ：This method was found to be simple and accurate.
出处
《中国药品标准》
CAS
2007年第3期66-68,共3页
Drug Standards of China
