摘要
目的探讨促性腺激素释放激素受体(GnRHR)和雌激素受体(ER)在子宫腺肌病在位内膜与异位内膜的表达、相关性及意义。方法收集51例行全子宫切除手术的子宫腺肌病患者,取其在位内膜为研究组A,异位内膜为研究组B,同期30份正常子宫内膜作为对照组。采用免疫组织化学方法测定各组内膜腺细胞中GnRHR与ER的表达,根据阳性率和表达强度进行量化评分(免疫组化评分),并做相关性分析。结果①研究组AGnRHR与ER的表达均显著高于研究组B和对照组,差异有统计学意义(P<0.05);②研究组A及正常子宫内膜中,GnRHR表达有周期性,分泌期显著高于增生期,差异有统计学意义(P<0.01),而在研究组B中增生期与分泌期相比较差异无统计学意义;③GnRHR与ER在各组内膜的表达水平无相关性(P>0.05)。结论GnRHR在子宫腺肌病在位内膜、异位内膜及正常子宫内膜中均有不同程度的表达;GnRHR在子宫腺肌病在位内膜及正常子宫内膜中分泌期表达显著高于增生期;推测促性腺激素释放激素(GnRH)可能通过其受体介导,对异位内膜细胞的种植和生长直接产生作用,在正常子宫内膜,则可能与受精卵着床及胚胎早期发育有关。
Objective To detect the expression of gonadotropin- realeasing hormone receptor （GnRHR） and estrogen receptor （ER） in eutopic and ectopic endometrium in adenomyosis and their relationship with clinical significance of adenomyosis. Methods Eutopic （study group A） and ectopic （study group 13） endometrium tissue specimens were obtained from 51 patients with adenomyosis, who underwent hysterectomy, and 30 normal endometrium tissue samples as control group. Expression of GnRHR and ER in endometrial cells were measured immunohistochemically. Results （1） Expressions of GnRHR and ER in group A were significantly higher than those in group B and normal control （P〈0.05）. （2） In group A and normal endometrium, expressions of GnRHR were in a periodical pattern and significantly higher in secretory endometrium than proliferative stage （ P〈0.01 ）. However, no difference between proliferative phase and secretory phase was found in group B. （3）The expression of GnRHR was not related to ER in each group （P〉0. 05）. Conclusions GnRHR and ER are detectable in normal, eutopic and ectopic endometrium from patients with adenomyosis. The expression pattern of GnRHR in endometrium indicates direct effect of GnRH on adenomyosis growth and implantation via its receptor, as well as physiologic function of GnRH related to oosperm imbeding and growth in the normal endometrium.
出处
《中国妇产科临床杂志》
2007年第4期273-276,281,共5页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
广州市卫生局项目基金(2006-YB-032)资助
