摘要
目的探讨荞麦花叶总黄酮(TFBFL)对去甲肾上腺素所致大鼠心肌肥厚的保护作用及其可能的机制。方法将大鼠随机分为6组(n=12):空白对照组,肥厚模型组,TFBFLⅠ、Ⅱ、Ⅲ组,和卡托普利治疗组(Cap组)。用去甲肾上腺素(NE)腹腔注射1.5mg/(kg·d),Bid,建立大鼠心肌肥厚模型,同时TFBFL和Cap灌胃给药100、200、400和50mg/(kg·d),o.d.,连续15d。观察心电图(ECG),左心室肥厚指数(LVWI:lvw/bw),和心重指数(HWI:hw/bw,lvw/hw)。采用比色法测定心室肌钙含量,用放免分析法检测血浆、心肌、肾脏血管紧张素Ⅰ(AngⅠ)生成率(反映肾素活性)和血管紧张素Ⅱ(AngⅡ)含量。结果与肥厚模型组比,TFBFL能剂量依赖性的改善心肌肥厚大鼠的ECG,降低LVWI和HWI,心肌钙含量和心肌AngⅡ含量,但对血浆肾脏AngⅡ含量无明显影响和心肌、血浆和肾脏AngⅠ生成率均无明显影响,且与阳性药物卡托普利组治疗效果相近。结论荞麦花叶总黄酮对大鼠心肌肥厚具有保护作用,其机制可能与拮抗心肌局部肾素-血管紧张素(RAS)系统有关。
[Objective] To study the protection of total flavones of buckwheat flower and leaf （TFBFL） on experimental cardiac hypertrophy induced by norepinephrine （NE） and its mechanism. [Methods] To divide the rats randomly into following 6 groups（n =12 per group）： （A）control, （B） hypertrophy, （C）TFBFL Ⅰ, （D）TFBFL Ⅱ, （E）TFBFLⅢ and（F）captopril. To establish the cardiac hypertrophy models of rats by ip of NE and observe the ECG, the left ventricular mass index （LVMI：lvw/bw） and the heart weight index（HWI：hw/bw,lvw/hw）. The angiotensin Ⅰ （Ang Ⅰ ） production rate （reflecting rennin activity） and angiotensin Ⅱ （Ang Ⅱ ） content of plasma, myocardium and kidney tissue were measured by radioinununoassay and myocardium Ca^2＋ by colorimetry. [Results] Compared with hypertrophy group, TFBFL could protect against cardiac hypertrophy of rats in a dose-dependent manner, such as improved ECG, LVWI and HWI, obviously decreased cardiac Ca^2＋ content, cardiac Ang Ⅱ content was decreased significantly without the same change of plasma and kidney tissue, and had similar relation with the treatment of captropril. [Conclusion] TFBFL has some profective effects on NE-indused cardiac hypertrophy in rats. The mechanism may be related to inhibition of local myocardium rennin- angiotensin -aldsteron system.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第11期1287-1289,1294,共4页
China Journal of Modern Medicine
关键词
荞麦花叶总黄酮
AngⅠ
AngⅡ
Ca^2+
心肌肥厚
total flavones of buckwheat flower and leaf（TFBFL）
cardiac hypertrophy
norepinephfine（NE）
an giotensin Ⅰ
angiotensin Ⅱ
Ca^2＋
