摘要
在pH为9．0的NH4Cl-NH3·H2O缓冲介质中，研究了Cu（Ⅱ）-苯基荧光酮（PF）-Triton X-100析相体系的最佳显色条件及其吸收光谱的分析特性．结果表明，Cu（Ⅱ）-PF-Triton X-100胶束溶液在95℃下加热1h，配合物即被Triton X-100相完全富集，其最大吸收峰为546nm，铜含量在0．5～8μg／5ml，符合比耳定律，表观摩尔吸光系数ε为4．7×10^4L·mol^-1·cm^-1；并尝试以氟化钾、酒石酸钾钠、柠檬酸钠为掩蔽剂，应用于食品中铜含量的测定，结果令人满意．
The optimum condition and the characteristics absorption spectrum of the phase-separation and chromomeric reaction of copper with 2,6,7-trihydroxy-9-phenyfluorone in the presence of Triton X-100 are studied. In pH 9.0 NH4Cl-NH3·H2O buffer medium, the complex can be enriched in Triton X-100 phase by heating for 1h at 95℃. The maximum absorption of the complex lies at 546nm. Beer＇s law is obeyed within 0.5-8 μg/5ml Cu（Ⅱ） with apparent molar absorptivity of 4.7×10^4 L·mol^-1·cm^-1. The proposed method has been applied to the determination of trace copper in food samples with satisfactory results in the presence of a mix occult reagent of potassium fluoride, sodium citrate and potassium sodium tartrate.
出处
《泉州师范学院学报》
2007年第2期65-67,74,共4页
Journal of Quanzhou Normal University
基金
福建省泉州市科技计划资助项目(Z200236)
关键词
苯基荧光酮
析相光度法
铜
phenyfluorone
phase-separation photometry
Cu （Ⅱ）
