摘要
目的建立猪肺表面活性物质及其冻干粉中7种磷脂组分的含量测定方法。方法采用硅胶柱,梯度洗脱,蒸发光散射检测器检测。结果磷脂酰甘油(PG)、磷脂酰乙醇胺(PE)、磷脂酰肌醇(PI)、磷脂酰丝氨酸(PS)、二棕榈酰磷脂酰胆碱(DP-PC)、鞘磷脂(SPH)、浴血磷脂酰胆碱(LPC)7种磷脂的线性回归方程的相关系数分别为0.999 0,0.998 4,0.999 0,0.999 3,0.999 8,0.998 8,0.998 1;日内精密度分别为2.1%,2.3%,2.8%,1.7%,1.5%,2.3%,2.3%;回收率分别为99.57%,101.03%,100.02%,100.17%,98.51%,100.65%,97.76%。结论本方法可同时测定猪肺表面活性物质及其冻干粉中7种磷脂组分的含量,专属性强、准确度高。
OBJECTIVE To develop a HPLC-ELSD method for the determination of seven phospholipid components in porcine pulmonary surfactant and its lyophilized powder. METHODS The samples were separated on a silica column with gradient elution and detected by evaporative light scattering detector. RESULTS The correlation coefficients of linear regression equation for phosphatidylglycerol （ PG）, phosphatidylethanolamine （ PE ）, phosphatidylinositol （ PI ）, phosphatidylserine （ PS ） , dipalmitoyl-phosphatidylcholine （ OPPC ）, sphingomyelin （ SPH ） and lysophophatidylcholine （ LPC ）, were 0. 999 0,0. 998 4,0. 999 0,0. 999 3,0. 999 8,0. 998 8 and 0. 998 1 respectively. The intraday precisions were 2. 1% ,2.3% ,2. 8% ,1.7% ,1.5% ,2. 3% and 2. 3% ,respectively. The recoveries were 99. 57%, 101.03% , 100. 02% , 100. 17% ,98. 51%, 100. 65% and 97.76% , respectively. CONCLUSION The method is accurate and specific, it can be used for the determination of seven phospholipid components in porcine pulmonary surfactant and its lyophilized powder simultaneously.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第12期938-942,共5页
Chinese Pharmaceutical Journal
