摘要
目的:应用大孔吸附树脂分离纯化大青叶有机酸部位。方法:大青叶以70%乙醇(pH=2)索氏提取,以邻氨基苯甲酸为考察指标,优选最佳吸附树脂并采用正交试验优化影响树脂吸附的重要因素,确定其合理工艺流程。结果:以HPD100型树脂对大青叶中有机酸组分的吸附与解吸性能较好,采用3BV(树脂床体积)60%乙醇易于洗脱;正交试验结果表明以药液中邻氨基苯甲酸浓度为0.18mg.mL-1、pH3.5及洗脱流速为2BV.h-1的参数组合应用效果良好,经济省时。结论:应用HPD100型大孔吸附树脂分离纯化大青叶中有机酸活性部位,其综合性能良好,具有一定的药用前景。
OBJECTIVE： To extract and purify organic acids with macroporous resins from Folium Isatidis. METHODS： Folium Isatidis was extracted with 70% aqueous ethanol（pH = 2） by Soxhlet extraction. The process was optimized by employing orthogonal test design with the content of anthranilic acid as the index. RESULTS： The resin HPD100 had much better adsorption capacity and desorption ratio by using 3 BV 60% aqueous ethanol to extract organic acids in Folium Isatidis than other resins. The results showed that HPD100 possessed the better ecomic under the concentration of sample 0.18mg·mL^-1, the condition of pH 3.5, and flowing rate of 2BV·h^-1. CONCLUSION： Macroporous resin HPD100 may be value of isolating and purifying the organic acids from Folium Isatidis.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第16期1214-1217,共4页
China Pharmacy
基金
国家自然科学基金资助项目(39800193)
关键词
大青叶
邻氨基苯甲酸
大孔吸附树脂
正交试验
分离纯化
Folium Isatidis
Anthranilic acids
Macroporous resin
Orthogonal design
Extraction and purification
