摘要
研究了食用菊花花瓣组织培养过程中不同植物生长调节剂和基因型对愈伤组织诱导和植株再生的影响,结果表明:附加2,4-D2.0 mg/L+KT 1.0 mg/L的MS培养基愈伤组织诱导率较高,愈伤组织只在MS+NAA 0.5 mg/L+6-BA 2.0 mg/L培养基上诱导出不定芽。不定芽转移到无植物生长调节剂的MS培养基上,可以得到再生植株。
The effect of plant growth regulators and genotypes on callus and plant regeneration from in vitro culture of edible chrysanthemum petal was studied.The results showed that the efficiency of callus in the MS medium supplemented with 2,4-D 2.0 mg/L and KT 1.0 mg/L were better than others.The adventitious bud only could be induced from callus in the MS medium supplemented with NAA 0.5 mg/L and 6-BA 2.0 mg/L.The regenerated plants were obtained,after the adventitious buds were transferred to MS medium without plant growth regulator.
出处
《安徽农业科学》
CAS
北大核心
2007年第16期4831-4832,共2页
Journal of Anhui Agricultural Sciences
基金
河北农业大学留学回国人员科研启动基金项目(2004-897)
关键词
食用菊花
花瓣培养
不定芽
植株再生
Edible chrysanthemum
Petal culture
Adventitious bud
Plant regeneration
