摘要
以柘树[Cudrania tricuspidata(Carr.)Bur.]的根为材料,经热水抽提、木瓜蛋白酶-Sevag法除蛋白、乙醇沉淀和DEAE-Sephadex A-50凝胶柱层析分离纯化,得到一种水溶性的柘树根多糖(CPS-0).采用HPLC、糖基组成分析、甲基化分析、GC、GC-MS、NMR(1H NMR,13C NMR及HMQC)、元素分析、UV和IR等技术对CPS-0的纯度、性质、组成和结构进行表征.结果表明,CPS-0仅含葡萄糖,分子量为4·6×103,主链由1,4-连接的α-D-葡萄糖残基组成,其侧链由末端及1,4-连接的葡萄糖残基构成,取代于主链分支点葡萄糖的6位,平均每10个葡萄糖残基组成的重复单元中含有1个分支.
A water-soluble polysaccharide （CPS-0） was obtained from the root of Cudrania tricuspidata （ Carr.） Bur. , by hot water extraction （70℃）, deproteination with enzymolysis and Sevag method, precipita-tion with ethanol, and fractionation through DEAE-Sephadex A-50 chromatography. The purity of CPS-0 was determined via HPLC and the structure was characterized with monosaccharide composition analysis, methyla-tion analysis, GC, GC-MS, NMR spectra（^1H NMR, ^13C NMR, HMQC）, UV, IR, and elemental analysis. The CPS-0 was found to contain glucose residue solely, with an average repeating unit of decasaccharide, ha-ving a backbone consisting of 1,4-1inked α-D-glucopyranosyl residues, to which the side chain consisting of terminal and 1,4-1inked α-D-glucopyranosyl residues was attached at position 6 of the branching residues.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2007年第6期1088-1091,共4页
Chemical Journal of Chinese Universities
基金
中国科学院'百人计划'人才引进启动基金资助
关键词
柘树根
多糖
分离纯化
结构表征
Root of Cudrania tricuspidata （Carr.） Bur.
Polysaccharide
Separation and purification
Struc-ture characterization
