摘要
目的：建立反相高效液相色谱法测定人血浆中盐酸伪麻黄碱浓度的方法。方法：以苯丙醇胺为内标，血浆样品经NaOH碱化，甲基叔丁基醚二次萃取后常温氮气挥干，100μl流动相溶解，50μl进样分析。色谱柱：ZORBAXSB-C18柱（4．6×150mm，5μm），柱温28℃；流动相：甲醇-水相（5：95，内含0．75％的三乙胺，以H，P04调节pH值至6．6），流速1．0ml·min^1，检测波长213nm。结果：盐酸伪麻黄碱在0．05～1mg·L^-1范围内线性关系良好，回归方程为Y=1．6151X-0．0804（r=0．998），相对回收率为94．4％～118．2％，日内、日间RSD均小于9．8％。结论：该方法简便、灵敏、稳定性好，可用于盐酸伪麻黄碱血药浓度的测定。
Objective：To establish a sensitive and specific RP-HPLC method for determination pseudoephodrine hydrochloride in human plasma. Methods： Human plasma with internal standard was extracted by methyl t-Butyl ether after being alkalinized. The supernatant was volatilized and then dissolved by 100μl mobile phase. The ZORBAX SB-C18 column（4.6×150mm, 5μm）was used with mobile phase of methanol-aqueous phase （5： 95, 0.75% triethylamine and regulate pH to 6.6 by H3 PO4 ）. The flow rate was 1.0 ml·min^-1 and the detection wave was 213 nm. Results：The linear range of pseudoephedrine was 0.05 -1mg·L^-1. The relative recovery were within 94.4% - 118.2%, the within-day and the between-day RSD were all less than 9.8%. Conclusion：This simple, specific and stable method is suitable for the determination of pseudoephedrine hydrochloride in human plasma.
出处
《军医进修学院学报》
CAS
北大核心
2007年第3期223-224,共2页
Academic Journal of Pla Postgraduate Medical School
