摘要
建立了高效液相色谱测定伪麻黄碱血药浓度的方法。血浆样品用乙醚-正己烷(1∶1V/V)提取,LiChrosorb 100CN为固定相,流动相为乙腈-甲醇-磷酸二氢钾-庚烷磺酸钠-重蒸水,检测波长为210 nm。线性范围为10.0～800.0 ng/ml,回收率>90%,日内、日间RSD<10%。12名男性受试者单剂量服用240 mg伪麻黄碱缓释片所得药代动力学参数为:T_(max)=8.58±2.47h;C_(max)=358.47±140.43mg/ml;t_(1/2)=10.27±3.04h;MRT=18.84±4.12h;AUC=7403.6±2166.6ng·h/ml。
A HPLC method was established for the determination of pseudoephedrine in human plasma. Pseudoephedrine was extracted from alikalined plasma to mixture of ether and n-hexane. A LiChrosorb 100 CN column was used and a UV detector was set at 210 nm. The mobile phase was a mixture of 50 ml acetonitrile, 8ml methanol, 4g potassium dihydrogenphosphate, 1g sodium heptanesulphonate and 400 ml water. The standard curve was linear over the concentration range of 10. 0～800. 0 ng/ml(r=0. 9997). The minimum detection concentration of pseudoephedrine in plasma was 10 ng/ml. The pharmacokinetic parameters of sustained-release formulation in 12 male volunteers were established as follows: T_(max)=8.58±2.47h; C_(max)=358.47±140.43ng/ml; t_(1/2)-10.27±3.04h; MRT=18.84±4.12h; AUC=7403.6±2166.6 ng·h/ml.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1996年第9期544-546,共3页
Journal of China Pharmaceutical University
