摘要
目的研究小鼠不同发育阶段肾小体细胞增殖的规律并探讨其作用。方法选择不同胚龄和生后日龄小鼠肾脏做常规光、电镜标本,并采用免疫组织化学方法测定不同发育阶段肾小体内PCNA阳性细胞表达率。结果PCNA免疫组织化学染色显示在Ⅰ和Ⅱ期肾小体内几乎所有细胞呈PCNA强阳性反应,Ⅲ、Ⅳ期肾小体内PCNA阳性细胞数逐渐减少,在Ⅴ期肾小体内PCNA阳性细胞少见。电镜观察所见:早期肾小体(Ⅰ、Ⅱ)所有细胞核分裂相机率均高,Ⅲ期肾小体中的内皮细胞、足细胞核分裂相机率高,Ⅳ期肾小体中只见到内皮细胞核分裂相。结论研究结果说明细胞增殖在小鼠肾小体发育、形成中有着重要的作用。
Objective To study the hales of cell proliferation in mouse glomenali at different stages of development. Methods The embryonic and postnatal mouse kidneys were choosed for study by light and electron microscopy, and PCNA immunohistochemistry method were used to observe proliferation within corpuscle at different stages of development. Results Nearly all cells in Ⅰ and Ⅱ stages had nuclear PCNA immunolabeling. The number of PCNA immunolabeling cells during Ⅲ and Ⅳ stages was gradually decreased, and to less in Ⅴ stage. Electron microscopy revealed that the mitotic activity was observed in all cells of Ⅰ and Ⅱ stages renal corpuscles. The mitotic phases of endothelial cells and podocytes could be seen in Ⅲ stage, but only endothelial mitotic activities in Ⅳ stage of renal corpuscle. Apoptotic activities of endothelial cell and Bowman＇s parietal cell were noted. Conclusions The results suggest that proliferation play an important role in the formation of glomeruli.
出处
《辽宁医学院学报》
CAS
2007年第2期34-37,共4页
Journal of Liaoning Medical University (LNMU) Bimonthly
关键词
肾小体
发育
细胞增殖
renal corpuscle
development
celluar proliferation
