摘要
在低温（１３０Ｋ）状态下用电子自旋共振（ＥＳＲ）技术直接检测到镉中毒小鼠肝组织产生的氧自由基（ｇ＝２．０２９），同时用脂类自由基捕集剂４－ＰＯＢＮ成功地捕集到肝细胞线粒体在体外与Ｃｄ２＋作用过程中产生的脂类自由基，得到的ＥＳＲ波谱的超精细分裂常数为：αＮ＝１．５６ｍＴ，αＨ＝０．２９８ｍＴ，反应体系中的丙二醛（ＭＤＡ）含量也明显升高。表明硒能有效地清除在体内镉中毒时肝组织产生的氧自由基和体外Ｃｄ２＋反应体系中产生的脂类自由基。
A electron spin resonant (ESR) signal of oxygen free radicals (g=2.029) could be directly detected at low temperature (130K) in the liver of mice treated with cadmium. Meanwhile, ESR spectra of spin adducts of 4 POBN with lipid free radicals (α N=1 56 mT,α H=0 298 mT) were obtained in a incubating system of mouse liver mitochondria with cadmium. In addition, MDA contents were significantly elevated in the incubating system. It was shown that selenium had a prominent scavenging effect on the oxygen free radicals in the liver and lipid free radicals generated in the incubating system of mitochondria with cadmium. Lipid peroxidation were also decreased.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1997年第1期50-53,共4页
Chinese Journal of Veterinary Science
基金
甘肃省自然科学基金
关键词
自由基
硒
镉
脂质过氧化
线粒体
小鼠
肝损伤
ESR
free radical
selenium
cadmium
lipid peroxidation
mitochondria
mouse
