摘要
目的探讨大鼠CO2气腹模型中不同压力CO2气腹对肾的影响及缺血预处理对肾的保护作用。方法建立大鼠CO2气腹模型,检测CO2气腹在不同压力和缺血预处理各组中肾氧化应激水平标志物:丙二醛(MDA)、谷胱甘肽还原酶(GSH)、超氧化物岐化酶(SOD)、一氧化氮(NO);对各组肾组织进行光镜下评分比较;并观察肾组织电镜结果。结果随着气腹压力的升高,肾氧化应激水平上升,光镜电镜组织学评价肾组织损伤程度增加,而缺血预处理能在一定程度上减轻气腹引起的肾损伤。结论CO2气腹对肾损伤与气腹压力高低有关,为了尽量减小气腹对肾的影响,应该采取气腹低压力或无气腹技术,而缺血预处理能对肾有保护作用。
Purpose To investigate the impact of different pressure of CO2 pneumoperitoneum on kidney and whether or not ischemic preconditioning before pneumoperitoneum could have some protective effect for kidney. Methods We established the CO2 pneumoperitoneum rat model, then made the determined the biochemical markers including malonaldehyde（MDA）, reduced glutathione hormone （GSH）, superoxide dismutase（SOD） ,and nitric oxide（NO） in the groups of different pressure and the group which accepted ischemic preconditioning. Besides, we made an microscopic evaluation of renal tissues and observed the electrenmicroscopic appearance among those groups. Results The oxidative stress of kidney rose with the increase of CO2 pneumoperitoneum pressure. The score of histological evaluation became higher with the increase of intrabdominal pressure. Ischemic preconditioning could reduce the oxidative injury caused by pneumoperitoneum. Conclusions Raising intraabdominal pressure leads to kidney injury in a pressure dependant manner. Clinically we should avoid using low pressure pneumoperitoneum and make a attempt to use a gasless technology. Ischemic preconditioning can make some protective effect on kidney injury.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2007年第3期351-354,363,共5页
Fudan University Journal of Medical Sciences
关键词
气腹
压力
自由基
缺血预处理
pneumoperitoneum
pressure
free radicals
ischemic preconditioning
