摘要
以6个草莓品种为试材,研究了影响草莓不定芽再生的各种因素,建立离体叶片高效再生系统。结果表明,外植体基因型、激素种类及配比、叶龄等是影响草莓再生的主要因子,其中‘鬼露甘’叶片最佳芽诱导培养基为MS+2.0 mg/L 6-BA+0.1 mg/L IBA,‘嫜姬’叶片愈伤组织的诱导以MS+3 mg/L 6-BA+0.2 mg/L 2,4-D较好,而且1周左右的暗培养可以防止外植体的褐化。芽伸长的最适培养基为MS+0.5 mg/L 6-BA+0.5 mg/L IBA,生根的最适培养基为MS+0.2 mg/L IBA,试管苗移栽后成活率为87%。
Six cultivars of strawberry （Fengxiang, Guilugan, Quanmingxiang, Zhangji, Hali, Xingxiang ） were investigated for factors affecting the regeneration of adventitious bud. The results indicated that the geno- type, hormones and seedling ages were the main factors affecting regeneration. The optimum shoot inducing medium of the leaves from Guilugan was MS＋2.0 mg/L 6-BA＋0. 1 mg/L IBA,the better medium for the induction of callus from the leaves of Zhangji was MS＋mg/L 6-BA3＋0.2 mg/L 2,4-D;the optimum medium for shoot elongation was MS＋0.5 mg/L 6-BA＋0.5 mg/L IBA,the optimum root inducing medium were MS＋0.2 mg/L IBA. The survival rate of the plantlets transplanted was 87%. Dark incubation for 1 week can reduce the browning of cultures. A highly efficient in vitro regeneration system from leaf explants was established.
出处
《西北植物学报》
CAS
CSCD
北大核心
2007年第5期919-925,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
福建省财政专项---福建省农业科学院科技创新团队建设基金资助(STIF-Y06)
关键词
草莓
离体再生体系
不定芽
strawberry
regeneration system
adventitious shoots
