摘要
目的探讨乙型肝炎病毒表面大蛋白(LHBs)用于临床乙型肝炎诊断的实验价值及与乙型肝炎病毒DNA定量的相关性。方法应用基因工程表达的乙型肝炎病毒表面大蛋白,采用ELISA技术共检测了510份HBsAg阳性血清标本中的LHBs,同时检测前S1抗原(PreS1Ag)及HBeAg,并应用荧光定量PCR技术平行检测HBVDNA。结果380份HBV DNA阳性血清标本中,361份(95.00%)LHBs检测阳性,PreS1Ag192份(50.50%)检测阳性;LHBs阳性率明显高于乙型肝炎前S1阳性率,两者差异有统计学意义(P<0.01);239份HBeAg阴性血清标本中,HBV DNA129份(53.93%)阳性,LHBs132份(55.23%)阳性,PreS1Ag77份(32.22%)阳性,LHBs阳性率与HBV DNA阳性率差异无统计学意义(P>0.05),均显著高于PreS1Ag;LHBs含量与HBV DNA拷贝数呈正相关性,相关系数r=0.946。结论LHBs是一个操作简便的可用于反映乙型肝炎病毒复制水平的敏感指标。
OBJECTIVE To review the clinical value of hepatitis B virus large surface protein（LHBs）used for diagnosis of the clinical hepatitis B patient and relativity with hepatitis B virus DNA. METHODS Genetic engineering technology was used to express the LHBs. Enzyme linked immunosorbent assay（ELISA） methods were used to examine the LHBs,PreS1 Ag and HBeAg and quantitative real-time PCR methods were used to detect the HBV DNA of 510 HBsAg-positive serum samples. RESULTS ①Significant difference of positive rate was found between LHBs （95.0% ,361/380） and PreS1Ag （50.50%, 192/380） in 380 HBV DNA-positive serum samples. ②No significant difference of positive rate was found between LHBs （55. 23%, 132/239） and HBV DNA （53.93%,129/239）; significant difference of positive rate was found between LHBs （55. 23%, 132/239） and PreS1Ag （32.22% ,77/239） in 239 HBeAg-negative serum samples. ③The content of LHBs and the HBV DNA copies performed a nice positive relativity （r=0. 946）. CONCLUSIONS Our results demonstrate that the LHBs is a sensitive index in reflecting hepatitis B virus replication level and its operation is convenient.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第6期624-626,共3页
Chinese Journal of Nosocomiology
