摘要
目的 观察栀子提取物ZG对单纯疱疹病毒1型（HSV-1）吸附过程中病毒吸附量的影响，以探讨其抗病毒作用机理。方法 采用异硫氰酸荧光素（fluorescein isothiocyanate，FTrc）作为荧光探针标记病毒，以肝素钠为对照，借助流式细胞仪，通过收集多种不同的加药方式后细胞表面的荧光强度，来考察病毒吸附过程中病毒吸附量的变化及栀子提取物ZG对此变化的影响。结果 单纯疱疹病毒1型在Hep-2细胞表面吸附存在饱和性、特异性；肝素钠3种加药方式均能明显降低病毒吸附量，先加药后吸附及吸附和加药同时进行两种方式，优于先吸附后加药的方式，其吸附抑制率分别为84．76％和82．02％；栀子提取物ZG3种加药方式均能明显降低病毒吸附量，其中以吸附和加药同时进行组最为显著，吸附抑制率为68．46％。结论 栀子提取物ZG可能是通过作用于病毒吸附的多个环节来达到抑制HSV-1的吸附作用。
Objective To observe the effect of Cardenia extract ZG on the adsorption quantity of HSV-1 and explore the mechanism of its antiviral activity.Methods With the fluorescein isothiocyanate （FTTC） as HSV-1 fluorescent probe and heparin sodium as the control,the flow cytometry （FCM） was adopted to study the effect of Garderda extract ZG on the adsorption quantity of HSV-1 on Hep-2 cell surface in groups with different drug administration methods.Resuits HSV/-1 adsorption quantity on Hep-2 surface had the character of saturation and specificity.Heparin soditun can prevent the attachment of HSV-1 which in accord with the abroad report.Among the three groups,G-F（heparin was added before the administration of F-HSV-1,i.e.HSV-1 virus marked by FITC） and F＋G （heparin and F-HSV-1 were added simultaneously） groups were better than F-G （F-HSV-1 was added before the administration of heparin） group and the inhibition ratio were 84.76% and 82.02%, respective- ly.In different Gardenia extract ZG administration methods, the attachment of HSV-1 on Hep-2 cells surface was prevented and the adsorption quantity was reduced, especially in the F＋Z（Cardenia extract ZG and F-HSV-1 were administrated simultaneously） group, the inhibition ratio was 68.46%. Conclusion Gardenia extract ZG may have effect on different elements of HSV-1 attachment.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2007年第4期321-325,共5页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金面上项目（30371754）
关键词
栀子提取物ZG
单纯疱疹病毒1型
病毒吸附量
流式细胞仪
异硫氰酸荧光素
Gardenia extractZG
Herpes simplex virus typel（HbV-1）
Adsorption quantity
Flow cy-tometer（FCM）
Fluorescein isothiocyanate（FITC）
