摘要
目的:观察碳酸化羟基磷灰石骨水泥与磷酸钙骨水泥的组织学反应差异,判定羟基磷灰石骨水泥中碳酸根存在的意义及其对组织相容性的影响。方法:实验于2000-01/2002-08在解放军总医院骨科研究所及医学动物实验中心完成。①碳酸化羟基磷灰石骨水泥的制备:以碳酸钙、磷酸三钙、磷酸氢钙等化学试剂为原材料,合成碳酸化羟基磷灰石骨水泥粉体。以磷酸氢二钠和磷酸二氢钠合成0.2mol/L磷酸钠缓冲液,作为固化液。固相/液相=1g/0.4mL。②细胞毒性实验:以磷酸钙骨水泥为对照,观察碳酸化羟基磷灰石骨水泥与骨髓基质细胞共培养后细胞的形态以及与材料表面的黏附性,并分别于2,4,6,8d用MTT法测量细胞的相对增殖率。③肌内埋植实验:选用成年新西兰兔8只,按随机数字表法分为实验组和对照组,每组4只。实验组将碳酸化羟基磷灰石预制成形,植入家兔背部肌肉组织内。对照组动物植入磷酸钙骨水泥。术后2,4,8,12周每组分别麻醉处死1只,观察材料周围组织的炎性反应和纤维包膜的形成情况。结果:纳入动物8只,均进入结果分析。①碳酸化羟基磷灰石骨水泥和磷酸钙骨水泥的浸提液分别与兔骨髓基质细胞共培养后,细胞的形态、与材料表面的黏附性以及细胞相对增殖率等无显著差别。②两种材料植入家兔背部肌肉内,材料周围形成的纤维组织包膜厚度均低于30μm,均未发现炎性细胞反应。植入12周时实验组碳酸化羟基磷灰石骨水泥的平均包膜厚度略低于对照组磷酸钙骨水泥(分别为22.7,26.1μm)。结论:碳酸化羟基磷灰石骨水泥和磷酸钙骨水泥均具有优秀的组织相容性,碳酸根的存在可以减少羟基磷灰石骨水泥的组织反应。
AIM： To investigate the difference of histological reaction between carbonated hydroxyapatite cement and calcium phosphate cement, and assess the significance and effect of carbonate ion in hydroxyapatite cement on histocompatibility.
METHODS： The experiment was performed in the Orthopedic Institute and animal experimental center of General Hospital of Chinese PLA from January 2000 to August 2002. （1） Preparation of carbonated hydroxyapatite cement （CHC）： The powder of cement was composed of calcium carbonate, tricalcium phosphate and calcium phosphate dibasic. 0.2 mol/L sodium phosphate buffer as solidification liquid was prepared by dibasic sodium phosphate and natrium biphosphoricum. Solid phase to liquid phase was 1 g to 0.4 mL. （2）Cytotoxicity test： With calcium phosphate cement as control, the morphological changes and material surface of the bone marrow stromal cells （BMSCs） after co-cultured with CHC were observed. The corresponding proliferation rate of BMSCs was detected at days 2, 4, 6 and 8 with MTT method. （3）Intramuscular implanting experiment： Eight adult New Zealand rabbits were selected and randomly divided into experiment group and control group with 4 rabbits in each group. Some standard rods of CHC were made and then implanted to the back of myoideum of the experiment group. The control group was implanted with calcium phosphate cement （CPC）. One rabbit of each group were sacrificed under anesthesia at 2, 4, 8 and 12 weeks, respectively after operation to observe the tissue inflammatory reaction around the materials and formation of fibrous capsules.
RESULTS： Totally 8 rabbits were involved in the result analysis. （1）After CHC and CPC leaching liquor were co-cultured with BMSCs, respectively, there were no significant differences among the morphous and proliferation of the cells and adhesivity of material surface. （2）After two kinds of materials were implanted into the back of myoideum of rabbits, the thickness of the fibrous capsules around both o
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第5期819-822,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助项目(39670731)~~
