摘要
为探讨c-kit蛋白在蝗虫卵子发生过程中的表达和调控机制,应用免疫组织化学和统计分析等方法对网翅蝗科（直翅目,蝗总科）3种蝗虫卵子发生过程中8个代表性阶段c-kit蛋白表达进行观测和比较,3种蝗虫分别为：绿牧草蝗 Omocestus viridulus（Linnaeus）,素色异爪蝗Euchorthippus unicolor（Ikonn.）和条纹异爪蝗Euchorthippus vittatus Zheng.结果显示蝗虫卵子发生第1～6阶段卵母细胞中有不同程度c-kit蛋白特异性表达,但随着卵黄发生的开始逐渐消失,而且3种蝗虫卵子发生过程中c-kit蛋白表达存在种间差异.以上结果提示c-kit蛋白在卵子发生中的表达暗示它参与和调控卵母细胞增殖与分化,此外c-kit蛋白表达种间差异说明在它的调控下不仅导致蝗虫卵子发生进程的迥异而且可能参与维系种间生殖隔离等机制.
In order to elucidate the expression and regulation of c- kit protein in oogenesis from grasshoppers, the special expressions of c-kit protein in oogenesis by using immunohistochemistry and biostatistics have been studied by collecting Omocestus viridulus （ Linnaeus ）, Euchorthippus unicolor （ Ikonn. ） and Euchorthippus vittatus Zheng in Siping area of Jilin Province, China. The results show that there are different expression levels of c- kit protein from the 1^st to 6^th stage in oogenesis but the positive granules gradually disappear with the following of vitellogenesis. The expressions of c-kit protein in oogenesis are significant differences among the three species grasshoppers. The data suggest that c- kit protein might play a crucial role in proliferation and differentiation as well as regulate differently developmental speed in oogenesis and even maintain species isolation etc.
出处
《动物分类学报》
CSCD
北大核心
2007年第2期343-349,共7页
Acta Zootaxonomica Sinica
基金
陕西省自然科学基金项目(2004C07)资助
