摘要
应用免疫组织化学和生物统计分析相结合的方法,对异翅负蝗Atractomorp haheteroptera Bei Bienko配子发生过程中c-kit特异表达特点和动态进行研究。结果表明,（1）精子发生过程中,精原细胞、初级精母细胞、次级精母细胞和成熟精子中均有不同程度的c-kit蛋白表达,精巢末端还有较粗大的阳性颗粒分布;（2）卵子发生过程中,第1-6阶段卵母细胞中有不同程度的c-kit蛋白特异性表达,但随着卵黄发生的开始逐渐消失;（3）此外,滤泡细胞、输卵管和受精囊的腺细胞中有c-kit蛋白颗粒的存在;（4）从c-kit蛋白季节动态变化看,精子发生和卵子发生中的阳性表达都随着时间的延续出现下降的态势。因此,c-kit蛋白的特异性表达提示该蛋白参与配子发生过程的阶段性调控,具有重要的生理作用。
By using immunohistochemistry and biostatistics, the special expression dynamics of c-kit protein in gametogenesis were studied by rearing Atractomorpha heteroptera Bei-Bienko in natural conditions. The results showed that： （1） In spermatogenesis, there were different expression levels of c-kit protein in spermatogonia, primary spermatocytes, socondd spermatocytes and mature sperms, and the biggest positive granules were found at the end of spermary; （2） In oogenesis, there were different expression levels of c-kit protein from the 1st to 6th stage of oocytes but the positive granules disappeared with the following vitellogenesis; （3） The c-kit positive granules were also found in follicular cells and the gland cells of oviduct and spermathecae; （4） The dynamic change of c-kit positive expression showed that it became stronger in young periods than in old periods not only in spermatogenesis but also in oogenesis. All in all, the special expression of c-kit protein implied that those proteins might participated in the stage regulation and play crucial role in gametogenesis.
出处
《昆虫知识》
CSCD
北大核心
2007年第2期205-210,共6页
Entomological Knowledge
基金
陕西省自然科学基金(2004C115)项目资助
关键词
异翅负蝗
配子发生
C-KIT蛋白
动态
Atractomorpha heteroptera, gametogenesis, c-kit protein, dynamics
