摘要
目的:探讨骨髓基质干细胞(BMSC)移植治疗实验性自身免疫性神经炎(experimentalautoimmuneneuritis,EAN)的疗效以及相应的作用机制。方法:用P0180-199多肽与弗氏完全佐剂的混合液免疫Lewis大鼠,建立EAN动物模型。治疗组在免疫后第10天,尾静脉回输荧光染料PKH26标记的BMSC(2×106个细胞/只),通过临床评估、免疫组化及ELISA等方法,研究了BMSC对EAN的治疗作用。结果:回输的BMSC能向脱髓鞘神经组织周围迁移,减轻脱髓鞘的病理改变和炎性细胞浸润。与对照组比较,治疗组CD4+和CD8+T细胞的浸润显著减少(P<0.05),血清中IFN-γ和TNF-α的水平明显降低(P<0.05),培养上清中IL-4的水平显著增加(P<0.05)。结论:BMSC静脉移植治疗EAN有一定的疗效。BMSC通过调节细胞因子的表达逆转Th1/Th2型细胞之间的失衡而发挥治疗作用,并能够抑制T细胞活化增殖。
AIM： To investigate the therapeutic effect of bone marrow stromal cells （BMSCs） transplantation on experimental autoimmune neuritis （EAN） and study the possible mechanism. METHODS： EAN model was established by immunizing Lewis rats with Po180-199 peptide and complete Freund＇s adjuvant （CFA）. In the therapy group, BMSCs （2 × 10^6 cells/rat） were marked with fluorochrome PKH26 and injected into the rats＇ caudal vein 10 d after immunization. The therapeutic effect of BMSC transplantation on EAN rats was investigated by clinical assessment, immunohistochemical staining, and ELISA, respectively. RESULTS： The injected BMSCs could migrate to the demyelinated nerve tissues, and the demyelination and inflammatory ir, filtration was relieved. The infiltration of CD4^＋ and CD8^＋ T cells and the sera level of IFN-γ and TNF-α were decreased significantly（ P 〈0.05）, whereas IL-4 level in the supernatant of cultured lymphocytes was increased （ P 〈 0. 05 ）. CONCLUSION： Certain therapeutic effect of BMSC transplantation on EAN was observed. BMSCs could reverse the disbalance of Th1/Th2 cells by regulating the cytokine expression and could inhibit the activation and proliferation of T cells.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2007年第1期52-55,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家"十五"科技攻关计划引导项目(2004DA754C)
黑龙江省卫生厅科技攻关项目(2005-29)
