摘要
采用盘状体剥离的方法获得蜈蚣藻Grateloupia filicima丝状体，在10、15、20℃条件下进行培养。结果表明：丝状体在20℃下形成的最快，数量最大，在15℃下生长最快；丝状体藻段与基质接触的细胞形成了盘状体，20℃是盘状体形成的适宜温度；丝状体藻段长度在50～250um内，切段长度越小，盘状体的形成率越高；盘状体在20℃下生长速度最快，直径达到200um左右时形成直立枝，不同温度对直立枝形成的影响不明显。采用丝状体切断的方法进行采苗，经过2个月的室内培养，生长出蜈蚣藻幼苗。
Filaments were induced the crusts of sea weed Grateloupia filicima peeled off from the substratum and incubated at water temperature of 10, 15 or 20 ℃. The most rapid formation and the maximal production of the filaments were observed at 20 ℃, while the best growth occurred at 15℃. The cells of the filament＇ s fragment adherent to the substratum gave rise to crusts. The optimal incubation temperature was 20 ℃ for the crusts production. From 50 um to 250 um, the shorter the fragment was, the more easily was the crusts produced. The crusts exhibited an optimal growth under 20 ℃ and the erect thalli were generated from them in their size of around 200 um. There was significant influence of temperature on the generation of erect thalli. The erect thalli had best growth at 20 ℃. The seedling incubation was conducted by chopped filaments into fragments, and germination was obtained in two months through indoor culture.
出处
《大连水产学院学报》
CSCD
北大核心
2007年第3期164-169,共6页
Journal of Dalian Fisheries University
基金
国家科技部"十一五"支撑计划资助项目(2006BAD09A01)
关键词
蜈蚣藻
盘状体
丝状体
诱导
Grateloupia filicima
crust
filament
induction
